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Literature summary extracted from

  • Wu, S.; Dou, W.; Wu, J.J.; Wang, J.J.
    Purification and partial characterization of glutathione S-transferase from insecticide-resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae) (2009), Arch. Insect Biochem. Physiol., 70, 136-150.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.18 dichlorvos the enzymes from different populations show different susceptibilities to dichlorvos Liposcelis paeta

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Ba2+
-
 Liposcelis paeta
2.5.1.18 Ca2+
-
 Liposcelis paeta
2.5.1.18 carbosulfan in vivo inhibition Liposcelis paeta
2.5.1.18 chlorpyrifos in vivo inhibition Liposcelis paeta
2.5.1.18 cypermethrin in vivo inhibition Liposcelis paeta
2.5.1.18 Ethacrynic acid specific inhibition Liposcelis paeta
2.5.1.18 Hg2+
-
 Liposcelis paeta
2.5.1.18 Mg2+
-
 Liposcelis paeta
2.5.1.18 Mn2+
-
 Liposcelis paeta
2.5.1.18 additional information the three populations of Liposcelis paeta show different susceptibilities to different insecticides, even after they are reared on diets consisting of 25% ethacrynic acid, no inhibition by Ba2+ Liposcelis paeta
2.5.1.18 Zn2+
-
 Liposcelis paeta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information purified enzyme, Michaelis-Menten kinetic analysis, overview Liposcelis paeta
2.5.1.18 0.68
-
 glutathione pH 7.5, 37°C, enzyme of Wuzhou population Liposcelis paeta
2.5.1.18 0.71
-
 glutathione pH 7.5, 37°C, enzymes of Hezhou and Nanyang population Liposcelis paeta
2.5.1.18 6.4
-
 1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Nanyang population Liposcelis paeta
2.5.1.18 16
-
 1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Wuzhou population Liposcelis paeta
2.5.1.18 223.6
-
 1-chloro-2,4-dinitrobenzene pH 7.5, 37°C, enzyme of Hezhou population Liposcelis paeta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 19420
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
2.5.1.18 20130
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
2.5.1.18 20430
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
2.5.1.18 23310
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
2.5.1.18 50790
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta
2.5.1.18 53140
-
 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene Liposcelis paeta the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Liposcelis paeta
-
 three field populations collected from Nanyang city of Henan Province, and Wuzhou and Hezhou cities of Guangxi Province, China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 native enzyme by glutathione affinity chromatography, three field populations from Nanyang, purification 42.5fold, Wuzhou, purification 64.5fold, and Hezhou purification 67.4fold Liposcelis paeta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.00065
-
 crude enzyme of Wuzhou population Liposcelis paeta
2.5.1.18 0.0012
-
 crude enzyme of Nanyang population Liposcelis paeta
2.5.1.18 0.0021
-
 crude enzyme of Hezhou population Liposcelis paeta
2.5.1.18 0.0494
-
 purified enzyme of Nanyang population Liposcelis paeta
2.5.1.18 0.0645
-
 purified enzyme of Wuzhou population Liposcelis paeta
2.5.1.18 0.0674
-
 purified enzyme of Hezhou population Liposcelis paeta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene the catalytic activity of purified GST from Nanyang city population with GSH is much higher than the activity of the enzyme from other populations, while the Wuzhou population reaches the highest in Vmax for substrate 1-chloro-2,4-dinitrobenzene Liposcelis paeta S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 additional information no activity with 1,2-dichloro-nitrobenzene Liposcelis paeta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 1 * 23310 + 1 * 20430, enzyme of Nanyang city population, SDS-PAGE, 1 * 53140 + 1 * 20130, enzyme of Wuzhou population, SDS-PAGE, 1 * 50790 + 1 * 19420, enzyme of Hezhou population, SDS-PAGE Liposcelis paeta

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Liposcelis paeta
2.5.1.18 GST
-
 Liposcelis paeta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 35
-
 enzyme from Nanyang population Liposcelis paeta
2.5.1.18 40
-
 enzymes from Wuzhou population and from Hezhou population Liposcelis paeta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 23 42 temperature profiles of enzyme from different populations, overview Liposcelis paeta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.3
-
 enzyme from Nanyang population Liposcelis paeta
2.5.1.18 7.5 8 enzyme from Wuzhou population Liposcelis paeta
2.5.1.18 8
-
 enzyme from Hezhou population Liposcelis paeta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 5.5 8.5 pH profiles of enzyme from different populations, overview Liposcelis paeta

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 additional information
-
 IC50 values for insecticides and metal ions for the enzymes from different populations, overview Liposcelis paeta additional information
2.5.1.18 0.000000395 0.000000842
-
 Liposcelis paeta Zn2+
2.5.1.18 0.000000873 0.00000585
-
 Liposcelis paeta Hg2+
2.5.1.18 0.00000181 0.00000203
-
 Liposcelis paeta Ethacrynic acid
2.5.1.18 0.000243 0.000583
-
 Liposcelis paeta Mg2+
2.5.1.18 0.00039 0.000978
-
 Liposcelis paeta cypermethrin
2.5.1.18 0.00132 0.00512
-
 Liposcelis paeta Mn2+
2.5.1.18 0.00158 0.0284
-
 Liposcelis paeta Ca2+
2.5.1.18 0.0056 0.0148 pH 7.5, 37°C Liposcelis paeta chlorpyrifos
2.5.1.18 0.055 0.806
-
 Liposcelis paeta carbosulfan