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Literature summary extracted from

  • Braker, J.D.; Hodel, K.J.; Mullins, D.R.; Friesen, J.A.
    Identification of hydrophobic amino acids required for lipid activation of C. elegans CTP:phosphocholine cytidylyltransferase (2009), Arch. Biochem. Biophys., 492, 10-16.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.15 additional information lipid activation Caenorhabditis elegans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 His-tagged version expressed in Sf9 cell using a baculovirus exression system Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 F260D part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 F260K part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 F260L part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 F260V part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 F269I part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 I256S part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 I257S part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 L246S part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 L246S/W249S/I256S/I257S part of a putative amphipathic alpha helix Caenorhabditis elegans
2.7.7.15 W249S part of a putative amphipathic alpha helix Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Caenorhabditis elegans Q3HKC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 TALON Co2+ metal affinity chromatography Caenorhabditis elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 10
-
 wild-type protein in the presence of 0.01 mM phosphatidylcholine:oleate Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Caenorhabditis elegans diphosphate + CDP-choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.15 CTP:phosphocholine cytidylyltransferase
-
 Caenorhabditis elegans