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Literature summary extracted from

  • Celano, L.; Gil, M.; Carballal, S.; Duran, R.; Denicola, A.; Banerjee, R.; Alvarez, B.
    Inactivation of cystathionine beta-synthase with peroxynitrite (2009), Arch. Biochem. Biophys., 491, 96-105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.22 truncated human CBS lacking 143 amino acids at the C-terminus is expressed as a fusion protein with glutathione S-transferase in the Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.22 DELTAC143 a truncated human CBS lacking 143 amino acids at the C-terminus is used to study the inactivation of cystathionine beta-synthase by peroxynitrite Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.22 peroxynitrite exposure to peroxynitrite does not modify bound pyridoxal 5'-phosphate but leads to nitration of Trp208, Trp43 and Tyr223 and alterations in the heme environment including loss of thiolate coordination, conversion to high-spin and bleaching, with no detectable formation of oxoferryl compounds nor promotion of one-electron processes Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.22 Fe3+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 45000
-
 the protein is an active and stable dimer of 45 kDa subunits Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Homo sapiens P35520
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.22 truncated human CBS lacking 143 amino acids at the C-terminus is purified as a fusion protein with glutathione S-transferase using the Escherichia coli expression vector pGEXCBSN. The protein is purified through affinity chromatography with glutathione sepharose and anion exchange chromatography. The glutathione Stransferase tag was cleaved by limited proteolysis using thrombin Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.22 9.8
-
-
 Homo sapiens

Subunits

EC Number Subunits Comment Organism
4.2.1.22 dimer 2 * 45000 Da, truncated human CBS lacking 143 amino acids at the C-terminus Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 CBS
-
 Homo sapiens
4.2.1.22 cystathionine beta-synthase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.22 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.22 8.3
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 heme
-
 Homo sapiens
4.2.1.22 pyridoxal 5'-phosphate
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.22 0.15
-
-
 Homo sapiens peroxynitrite