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Literature summary extracted from

  • Czekster, C.M.; Neto, B.A.; Lapis, A.A.; Dupont, J.; Santos, D.S.; Basso, L.A.
    Steady-state kinetics of indole-3-glycerol phosphate synthase from Mycobacterium tuberculosis (2009), Arch. Biochem. Biophys., 486, 19-26.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.48 drug development indole-3-glycerol phosphate synthase of Mycobacterium tuberculosis is an attractive target for new anti-Tuberculosis drug development Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.48 expression in Escherichia coli BL21(DE3) STAR Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.055
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.48 30000
-
 gel-filtration chromatography Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.48 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate Mycobacterium tuberculosis
-
 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.48 chromatographic purification, Phenyl Sepharose High Sub FF column, ultrafiltration membrane (molecular weight cutoff) of 10,000 Da, Sephacryl S-100 column Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 Mycobacterium tuberculosis 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.48 monomer 1 * 30000 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.48 IGPS
-
 Mycobacterium tuberculosis
4.1.1.48 indole-3-glycerol phosphate synthase
-
 Mycobacterium tuberculosis
4.1.1.48 MtIGPS
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.48 15 35 activation energy value of 8.4 kcal/mol, the linearity of the Arrhenius plot demonstrates that there is no change in the rate-limiting step in the temperature range employed Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.48 0.16
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.48 4.5 8
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.48 2.9
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, pH 7.5 Mycobacterium tuberculosis