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Literature summary extracted from

  • Ducati, R.G.; Santos, D.S.; Basso, L.A.
    Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis (2009), Arch. Biochem. Biophys., 486, 155-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.012
-
 guanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.015
-
 2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.029
-
 2-amino-6-mercapto-7-methylpurine ribonucleoside
-
 Mycobacterium tuberculosis
2.4.2.1 0.033
-
 7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.04
-
 Inosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.068
-
 2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.427
-
 phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.427
-
 phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 0.583
-
 phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 1.131
-
 phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 1.345
-
 phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 2.349
-
 phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Mycobacterium tuberculosis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate steady-state ordered bi bi kinetic mechanism, in which phosphate binds first followed by 2'-deoxyguanosine, and ribose 1-phosphate dissociates first followed by guanine. A general acid is essential for both catalysis and 2'-deoxyguanosine binding, and deprotonation of a group abolishes phosphate binding. Product release contributes to the rate-limiting step Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2'-deoxyguanosine + phosphate
-
 Mycobacterium tuberculosis guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 2'-deoxyinosine + phosphate
-
 Mycobacterium tuberculosis 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
-
 ?
2.4.2.1 2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
-
 Mycobacterium tuberculosis alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
 ?
2.4.2.1 7-methylguanosine + phosphate
-
 Mycobacterium tuberculosis 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 7-methylinosine + phosphate
-
 Mycobacterium tuberculosis 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Mycobacterium tuberculosis guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Mycobacterium tuberculosis hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 additional information enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine Mycobacterium tuberculosis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 3.5
-
 phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 4
-
 guanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 4.9
-
 phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 4.9
-
 phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 5.1
-
 2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 5.4
-
 Inosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 6.9
-
 phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 7.5
-
 2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 13.3
-
 phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 17.3
-
 2-amino-6-mercapto-7-methylpurine ribonucleoside
-
 Mycobacterium tuberculosis
2.4.2.1 17.9
-
 phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 18
-
 7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 2.1
-
 phosphate cosubstrate 2'-deoxyguanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 2.6
-
 phosphate cosubstrate guanosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 6.1
-
 phosphate cosubstrate 2'-deoxyinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 31
-
 phosphate cosubstrate 7-methylinosine, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 42
-
 phosphate cosubstrate 2-amino-6-mercapto-7-methylpurine ribonucleoside, pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 110
-
 2'-deoxyinosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 140
-
 Inosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 330
-
 guanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 340
-
 2'-deoxyguanosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 550
-
 7-Methylinosine pH 7.0, 25°C Mycobacterium tuberculosis
2.4.2.1 600
-
 2-amino-6-mercapto-7-methylpurine ribonucleoside
-
 Mycobacterium tuberculosis
2.4.2.1 8400
-
 phosphate cosubstrate inosine, pH 7.0, 25°C Mycobacterium tuberculosis