BRENDA - Enzyme Database

Effect of deletion of a plant like pentapeptide insert on kinetic, structural and immunological properties of enolase from Plasmodium falciparum

Vora, H.K.; Shaik, F.R.; Pal-Bhowmick, I.; Mout, R.; Jarori, G.K.; Arch. Biochem. Biophys. 485, 128-138 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Plasmodium falciparum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
deletion of a plant like pentapeptide insert 104EWGWS108 in a highly conserved surface loop of the protein results in about 100fold decrease in kcat/Km and causes dissociation of dimeric form into monomers
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.04
-
2-phospho-D-glycerate
recombinant, dimeric enzyme; recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
155000
-
gel filtration
Plasmodium falciparum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Plasmodium falciparum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
glutathione-Sepharose beads chromatography, gel filtration
Plasmodium falciparum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701914
Plasmodium falciparum
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
gel filtration
Plasmodium falciparum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.11
44.7
-
dimeric recombinant enolase shows two transitions which corresponds to dissociation of dimer in to monomer (Tm of 44.7C)
Plasmodium falciparum
4.2.1.11
58.1
-
dimeric recombinant enolase shows two transitions which corresponds to unfolding of monomer (Tm of 58.1C)
Plasmodium falciparum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
20.6
-
2-phospho-D-glycerate
recombinant, dimeric enzyme
Plasmodium falciparum
4.2.1.11
22.2
-
2-phospho-D-glycerate
recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Plasmodium falciparum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
deletion of a plant like pentapeptide insert 104EWGWS108 in a highly conserved surface loop of the protein results in about 100fold decrease in kcat/Km and causes dissociation of dimeric form into monomers
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.04
-
2-phospho-D-glycerate
recombinant, dimeric enzyme; recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
155000
-
gel filtration
Plasmodium falciparum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
glutathione-Sepharose beads chromatography, gel filtration
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701914
Plasmodium falciparum
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
gel filtration
Plasmodium falciparum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.11
44.7
-
dimeric recombinant enolase shows two transitions which corresponds to dissociation of dimer in to monomer (Tm of 44.7C)
Plasmodium falciparum
4.2.1.11
58.1
-
dimeric recombinant enolase shows two transitions which corresponds to unfolding of monomer (Tm of 58.1C)
Plasmodium falciparum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
20.6
-
2-phospho-D-glycerate
recombinant, dimeric enzyme
Plasmodium falciparum
4.2.1.11
22.2
-
2-phospho-D-glycerate
recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
520
-
2-phospho-D-glycerate
recombinant, dimeric enzyme
Plasmodium falciparum
4.2.1.11
560
-
2-phospho-D-glycerate
recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
520
-
2-phospho-D-glycerate
recombinant, dimeric enzyme
Plasmodium falciparum
4.2.1.11
560
-
2-phospho-D-glycerate
recombinant, His-tagged, hexameric enzyme
Plasmodium falciparum