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Literature summary extracted from

  • Vora, H.K.; Shaik, F.R.; Pal-Bhowmick, I.; Mout, R.; Jarori, G.K.
    Effect of deletion of a plant like pentapeptide insert on kinetic, structural and immunological properties of enolase from Plasmodium falciparum (2009), Arch. Biochem. Biophys., 485, 128-138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 additional information deletion of a plant like pentapeptide insert 104EWGWS108 in a highly conserved surface loop of the protein results in about 100fold decrease in kcat/Km and causes dissociation of dimeric form into monomers Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.04
-
 2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
4.2.1.11 0.04
-
 2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 155000
-
 gel filtration Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 glutathione-Sepharose beads chromatography, gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Plasmodium falciparum phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 homodimer gel filtration Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Plasmodium falciparum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.11 44.7
-
 dimeric recombinant enolase shows two transitions which corresponds to dissociation of dimer in to monomer (Tm of 44.7°C) Plasmodium falciparum
4.2.1.11 58.1
-
 dimeric recombinant enolase shows two transitions which corresponds to unfolding of monomer (Tm of 58.1°C) Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.11 20.6
-
 2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
4.2.1.11 22.2
-
 2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.11 520
-
 2-phospho-D-glycerate recombinant, dimeric enzyme Plasmodium falciparum
4.2.1.11 560
-
 2-phospho-D-glycerate recombinant, His-tagged, hexameric enzyme Plasmodium falciparum