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Literature summary extracted from

  • Todorova, N.A.; Schwarz, F.P.
    Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase (2008), Arch. Biochem. Biophys., 480, 122-131.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 9-benzylguanine isothermal titration calorimetry study. At 25°C, the tighter 9-benzylguanine binding reaction goes from an enthalpically-driven reaction in the absence of phosphate to an entropically-driven reaction at 10 mM phosphate, and the enthalpically-driven nature of the binding reaction is restored at 75 mM phosphate. Bound phosphate affects the interactions of the side-chains with the ribose catalytic site. 9-Benzylguanine interacts with Phe159 from an adjacent subunit Homo sapiens
2.4.2.1 acyclovir isothermal titration calorimetry study. At 25°C and with an increase in the phosphate concentration from 0 to 50 mM, acyclovir binding becomes more entropically-driven Homo sapiens
2.4.2.1 ganciclovir isothermal titration calorimetry study. At 25°C and with an increase in the phosphate concentration from 0 to 50 mM, ganciclovir binding becomes more enthalpically-driven Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 9.2
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 12.5
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 13
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 13.9
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 16.3
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 39.3
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 12
-
 presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 20
-
 presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 41
-
 presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 46
-
 presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 47
-
 presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 139
-
 presence of 50 mM phosphate, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methylguanosine + phosphate
-
 Homo sapiens 7-methylguanine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 monomer partial dissociation of the purine nucleotide phosphates trimer into monomers below 60 nM Homo sapiens
2.4.2.1 trimer partial dissociation of the purine nucleotide phosphates trimer into monomers below 60 nM Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 6.23
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 9.2
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 20.4
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 23
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 24.7
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 73.1
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 240
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 488
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 1548
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 1674
-
 7-methylguanosine presence of 100 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 2256
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens
2.4.2.1 5860
-
 7-methylguanosine presence of 50 mM phosphate, pH 7.0 Homo sapiens