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Literature summary extracted from

  • Bohlmann, J.; Phillips, M.; Ramachandiran, V.; Katoh, S.; Croteau, R.
    cDNA cloning, characterization, and functional expression of four new monoterpene synthase members of the Tpsd gene family from grand fir (Abies grandis) (1999), Arch. Biochem. Biophys., 368, 232-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.16 expression in Escherichia coli Abies grandis
4.2.3.52 expression in Escherichia coli Abies grandis
4.2.3.113 expression in Escherichia coli Abies grandis
4.2.3.117 expression in Escherichia coli Abies grandis
4.2.3.119 expression in Escherichia coli Abies grandis
4.2.3.120 expression in Escherichia coli Abies grandis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.117 70750
-
 x * 70750, calculated Abies grandis
4.2.3.119 70750
-
 x * 70750, calculated Abies grandis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.16 Abies grandis Q9M7D0
-
-
4.2.3.52 Abies grandis Q9M7D1
-
-
4.2.3.113 Abies grandis Q9M7D0
-
-
4.2.3.117 Abies grandis Q948Z0
-
-
4.2.3.119 Abies grandis Q948Z0
-
-
4.2.3.119 Abies grandis Q9M7D0
-
-
4.2.3.120 Abies grandis Q9M7D0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.3.16 proteolytic modification sequence contains a putative plastid targeting signal Abies grandis
4.2.3.113 proteolytic modification sequence contains a putative plastid targeting signal Abies grandis
4.2.3.117 proteolytic modification sequence contains a putative N-terminal targeting peptide Abies grandis
4.2.3.119 proteolytic modification sequence contains a putative N-terminal targeting peptide Abies grandis
4.2.3.119 proteolytic modification sequence contains a putative plastid targeting signal Abies grandis
4.2.3.120 proteolytic modification sequence contains a putative plastid targeting signal Abies grandis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.16 geranyl diphosphate
-
 Abies grandis (-)-limonene + diphosphate 42% terpinolene, 18% (-)-alpha-pinene, 11% (-)-limonene, 10% (-)-beta-pinene plus several minor products ?
4.2.3.16 additional information entire product set is derived in stereochemically consistent fashion via (-)-3S-linalyl diphosphate as intermediate Abies grandis ?
-
 ?
4.2.3.52 geranyl diphosphate soluble enzyme preparations from Escherichia coli BL21(DE3)/pSAG8(M), expressing (-)-beta-phellandrene synthase convert geranyl diphosphate to four products. beta-phellandrene (52%) as the major olefin, and lesser amounts of (1S,5S)-beta-pinene (34%), (1S,5S)-apinene (8.5%), and (4S)-limonene (6%) are identified. The stereochemistry of beta-phellandrene is not confirmed directly. Nevertheless, stereochemical considerations based on the established absolute configuration of the coproducts, and the natural occurrence of (4S)-beta-phellandrene in the turpentine, suggests that the biosynthetic product is the mechanistically related (4S)-antipode derived via the (3S)-linalyl diphosphate intermediate. The product of the ag8 gene is therefore designated (4S)-beta-phellandrene synthase Abies grandis (4S)-beta-phellandrene + diphosphate
-
 ?
4.2.3.113 geranyl diphosphate
-
 Abies grandis terpinolene + diphosphate 42% terpinolene, 18% (-)-alpha-pinene, 11% (-)-limonene, 10% (-)-beta-pinene plus several minor products ?
4.2.3.113 additional information entire product set is derived in stereochemically consistent fashion via (-)-3S-linalyl diphosphate as intermediate Abies grandis ?
-
 ?
4.2.3.117 geranyl diphosphate
-
 Abies grandis (-)-camphene + diphosphate products are 54% (-)-(1S,4R)-camphene, followed by 32% (-)-(1S,5S)-alpha-pinene and 7% (-)-(4S)-limonene ?
4.2.3.119 geranyl diphosphate
-
 Abies grandis (-)-alpha-pinene + diphosphate 42% terpinolene, 18% (-)-alpha-pinene, 11% (-)-limonene, 10% (-)-beta-pinene plus several minor products ?
4.2.3.119 geranyl diphosphate
-
 Abies grandis (-)-alpha-pinene + diphosphate products are 54% (-)-(1S,4R)-camphene, followed by 32% (-)-(1S,5S)-alpha-pinene and 7% (-)-(4S)-limonene. (+)-alpha-pinene is produced to about 5% of (-)-alpha-pinene ?
4.2.3.119 additional information entire product set is derived in stereochemically consistent fashion via (-)-3S-linalyl diphosphate as intermediate Abies grandis ?
-
 ?
4.2.3.120 geranyl diphosphate
-
 Abies grandis (-)-beta-pinene + diphosphate 42% terpinolene, 18% (-)-alpha-pinene, 11% (-)-limonene, 10% (-)-beta-pinene plus several minor products ?
4.2.3.120 additional information entire product set is derived in stereochemically consistent fashion via (-)-3S-linalyl diphosphate as intermediate Abies grandis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.117 ? x * 70750, calculated Abies grandis
4.2.3.119 ? x * 70750, calculated Abies grandis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.16 ag9
-
 Abies grandis
4.2.3.52 (-)-(4S)-beta-phellandrene synthase
-
 Abies grandis
4.2.3.52 (-)-beta-phellandrene synthase
-
 Abies grandis
4.2.3.113 ag9
-
 Abies grandis
4.2.3.117 ag6
-
 Abies grandis
4.2.3.117 camphene synthase
-
 Abies grandis
4.2.3.119 ag6
-
 Abies grandis
4.2.3.119 ag9
-
 Abies grandis
4.2.3.119 camphene synthase
-
 Abies grandis
4.2.3.120 ag9
-
 Abies grandis