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Literature summary extracted from

  • Savage, T.J.; Ichii, H.; Hume, S.D.; Little, D.B.; Croteau, R.
    Monoterpene synthases from gymnosperms and angiosperms: stereospecificity and inactivation by cysteinyl- and arginyl-directed modifying reagents (1995), Arch. Biochem. Biophys., 320, 257-265.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.120 additional information enzyme is inactivated by thiol-modifying agents, coincubation with substrate and metal-ion cofactor does not protect Pinus contorta
4.2.3.120 Phenylglyoxal inactivation, coincubation with substrate and metal-ion cofactor reduces the rate of inactivation by 10-fold Pinus contorta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.120 0.001
-
 (3S)-linalyl diphosphate pH 7.0, 31°C Pinus contorta
4.2.3.120 0.0025
-
 geranyl diphosphate pH 7.0, 31°C Pinus contorta
4.2.3.120 0.0032
-
 (3R)-linalyl diphosphate pH 7.0, 31°C Pinus contorta

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.52 Pinus contorta
-
-
-
4.2.3.120 Pinus contorta
-
 isoform cyclase IV
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.120 (3R)-linalyl diphosphate
-
 Pinus contorta myrcene + diphosphate
-
 ?
4.2.3.120 (3S)-linalyl diphosphate
-
 Pinus contorta (-)-beta-pinene + diphosphate 63% (-)-beta-pinene, 25.8% (+-)carene, and 6.7% alpha-pinene in almost racemic mixture.Products are exactly the same as with geranyl diphosphate ?
4.2.3.120 geranyl diphosphate
-
 Pinus contorta (-)-beta-pinene + diphosphate 63% (-)-beta-pinene, 25.8% (+-)carene, and 6.7% alpha-pinene in almost racemic mixture. (+)-alpha-pinene arises from the rare isomerization of geranyl diphosphate to (3R)-linalyl diphosphate ?