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Literature summary extracted from

  • Nagata, T.; Morita, H.; Akizawa, T.; Pan-Hou, H.
    Development of a transgenic tobacco plant for phytoremediation of methylmercury pollution (2010), Appl. Microbiol. Biotechnol., 87, 781-786.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.99.1.2 environmental protection a new transgenic tobacco plant for phytoremediation of CH3Hg+ pollution: The new ppk/merT/merB-transgenic tobacco plant, which contains the integrated bacterial merB gene encoding MerB, has the ability to tolerate and accumulate high levels of Hg2+ inside the plant cells from simulated soils, probably via a chelation mechanism of polyP with Hg2+, without releasing mercury vapor into the atmosphere Pseudomonas sp. K-62

Protein Variants

EC Number Protein Variants Comment Organism
4.99.1.2 additional information The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens, the tobacco callus shows more resistance to CH3Hg+ and accumulated significantly more mercury from CH3Hg+-containing medium than does the ppk/merT-transgenic and wild-type tobaccos Pseudomonas sp. K-62

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.99.1.2 CH3Hg+ the growth rates of both the wild-type and ppk/merT-transgenic tobacco callus are largely inhibited by CH3Hg+ in a dose-dependent manner Pseudomonas sp. K-62

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.2 Pseudomonas sp. K-62 O07303 The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.2 CH3Hg+ + H+ the growth rates of both the wild-type and ppk/merTtransgenic tobacco callus are largely inhibited by CH3Hg+ in a dose-dependent manner Pseudomonas sp. K-62 Hg2+ + CH4
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Synonyms

EC Number Synonyms Comment Organism
4.99.1.2 merB
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 Pseudomonas sp. K-62
4.99.1.2 organomercurial lyase
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 Pseudomonas sp. K-62