Any feedback?
Literature summary extracted from
- Cloning, overexpression, and characterization of recombinant heparinase III from Bacteroides stercoris HJ-15 (2010), Appl. Microbiol. Biotechnol., 86, 879-890.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.8 | His-tagged version expressed in Escherichia coli BL21(DE3) | Bacteroides stercoris |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.2.8 | C352A | 87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) | Bacteroides stercoris |
| 4.2.2.8 | C474A | 32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) | Bacteroides stercoris |
| 4.2.2.8 | C577A | 98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) | Bacteroides stercoris |
| 4.2.2.8 | C624A | 24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) | Bacteroides stercoris |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.8 | Cu2+ | 1 mM: 0% activity | Bacteroides stercoris |  |
| 4.2.2.8 | Pb2+ | 1 mM: 0% activity | Bacteroides stercoris | |
| 4.2.2.8 | Zn2+ | 1 mM: 0% activity | Bacteroides stercoris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.8 | additional information | - |
heparan sulfate | 0.08808 mg/ml | Bacteroides stercoris | |
| 4.2.2.8 | additional information | - |
heparin | 0.42275 mg/ml | Bacteroides stercoris | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.8 | Ba2+ | 1 mM: 109% activity | Bacteroides stercoris | |
| 4.2.2.8 | Ca2+ | 1 mM: 110% activity | Bacteroides stercoris | |
| 4.2.2.8 | additional information | optimal condition: 350 mM NaCl | Bacteroides stercoris |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | 74930 | - |
x * 74930, calculated for mature protein | Bacteroides stercoris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.8 | Bacteroides stercoris | C7EXL6 | - |
- |
| 4.2.2.8 | Bacteroides stercoris HJ-15 | C7EXL6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.8 | immobilized metal ion affinity chromatography | Bacteroides stercoris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | additional information | - |
no activity with acharan sulfate, chondroitin sulfate A, and chondroitin sulfate C | Bacteroides stercoris |
| 4.2.2.8 | 1.7 | - |
hyaluronic acid as substrate | Bacteroides stercoris |
| 4.2.2.8 | 21.46 | - |
heparin as substrate | Bacteroides stercoris |
| 4.2.2.8 | 32.3 | - |
heparan sulfate (porcine intestine) as substrate | Bacteroides stercoris |
| 4.2.2.8 | 51.81 | - |
heparan sulfate (bovine kidney) as substrate | Bacteroides stercoris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.8 | heparan sulfate | - |
Bacteroides stercoris | n alpha-deltaUA-glcNAc | major product | ? | |
| 4.2.2.8 | heparan sulfate | - |
Bacteroides stercoris HJ-15 | n alpha-deltaUA-glcNAc | major product | ? | |
| 4.2.2.8 | heparin | - |
Bacteroides stercoris | ? | - |
? | |
| 4.2.2.8 | heparin | - |
Bacteroides stercoris HJ-15 | ? | - |
? | |
| 4.2.2.8 | hyaluronic acid | - |
Bacteroides stercoris | ? | - |
? | |
| 4.2.2.8 | hyaluronic acid | - |
Bacteroides stercoris HJ-15 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.8 | ? | x * 74930, calculated for mature protein | Bacteroides stercoris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.8 | heparinase III | - |
Bacteroides stercoris |
| 4.2.2.8 | heparitinase | - |
Bacteroides stercoris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | 45 | - |
- |
Bacteroides stercoris |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | 42 | 48 | activity above 90% | Bacteroides stercoris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | additional information | - |
about 20% activity after 1 h at 45°C | Bacteroides stercoris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | 6.6 | - |
- |
Bacteroides stercoris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.8 | 6 | 8 | - |
Bacteroides stercoris |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.2.8 | Bacteroides stercoris | calculated for mature protein | - |
8.65 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
