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Literature summary extracted from
- A novel acid-stable, acid-active beta-galactosidase potentially suited to the alleviation of lactose intolerance (2010), Appl. Microbiol. Biotechnol., 86, 517-524.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.23 | the purified enzyme retains 68% residual activity after in vitro exposure to simulated gastric conditions (pepsin at pH 2.0) for 2 h | Aspergillus niger |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.23 | extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 123000 | - |
glycosylated enzyme, gel filtration | Aspergillus niger |
| 3.2.1.23 | 129000 | - |
1 * 129000, glycosylated enzyme, SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Aspergillus niger | P29853 | strain DSM 22593 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | DEAE-Sepharose column chromatography, Sephacryl S200 gel filtration, phenyl-agarose column chromatography, and PBE 94 chromatofocusing column chromatography | Aspergillus niger |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 1.3 | - |
crude enzyme, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C | Aspergillus niger |
| 3.2.1.23 | 69.3 | - |
after 53.3fold purification, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | 6% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 2-nitrophenol + beta-D-galactopyranose | - |
? |  |
| 3.2.1.23 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | 100% activity | Aspergillus niger | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 3.2.1.23 | 4-nitrophenyl alpha-L-arabinopyranoside + H2O | 42% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| 3.2.1.23 | 4-nitrophenyl beta-D-fucopyranoside + H2O | 3% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 3.2.1.23 | 4-nitrophenyl beta-D-galactopyranoside + H2O | 81% activity compared to 4-nitrophenyl alpha-D-galactopyranoside | Aspergillus niger | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.23 | 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O | - |
Aspergillus niger | 5-bromo-4-chloroindol + beta-D-galactose | - |
? | |
| 3.2.1.23 | lactose + H2O | - |
Aspergillus niger | D-galactose + D-glucose | - |
? | |
| 3.2.1.23 | additional information | no activity towards 4-nitrophenyl alpha-D-glucopyranoside and 4-nitrophenyl alpha-D-mannopyranoside | Aspergillus niger | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | monomer | 1 * 129000, glycosylated enzyme, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | lactase | - |
Aspergillus niger |
| 3.2.1.23 | lactase phlorizin hydrolase | - |
Aspergillus niger |
| 3.2.1.23 | LPH | - |
Aspergillus niger |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 65 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 50 | 75 | - |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 60 | 80 | a sharp drop of activity occurs above 60°C, no activity is detected at 80°C | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 2 | 4 | - |
Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 4 | 8 | a sharp drop of activity occurs above pH 4.0, about 35% residual activity at pH 4.5-5.5, about 10% residual activity at pH 6.5, no activity is detected at pH 8.0 and above | Aspergillus niger |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.23 | Aspergillus niger | glycosylated enzyme, isoelectric focusing | - |
4.7 |
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Release 2023.1 (January 2023)
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