EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.3.3 | biotechnology | efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 | Escherichia coli |
5.1.3.8 | biotechnology | efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 | Synechocystis sp. PCC 6803 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.3 | 2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production | Escherichia coli |
5.1.3.8 | 2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production | Synechocystis sp. PCC 6803 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 33000 | - |
determined by SDS-PAGE | Escherichia coli |
5.1.3.8 | 40000 | - |
determined by SDS-PAGE | Synechocystis sp. PCC 6803 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-acetyl-D-neuraminic acid | Escherichia coli | - |
N-acetyl-D-mannosamine + pyruvate | - |
r | |
5.1.3.8 | N-acetyl-D-mannosamine | Synechocystis sp. PCC 6803 | - |
N-acetyl-D-glucosamine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.3 | Escherichia coli | - |
- |
- |
5.1.3.8 | Synechocystis sp. PCC 6803 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | additional information | - |
after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-acetyl-D-neuraminic acid | - |
Escherichia coli | N-acetyl-D-mannosamine + pyruvate | - |
r | |
5.1.3.8 | N-acetyl-D-mannosamine | - |
Synechocystis sp. PCC 6803 | N-acetyl-D-glucosamine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.3 | N-acetyl-d-neuraminic acid aldolase | - |
Escherichia coli |
4.1.3.3 | Neu5Ac aldolase | - |
Escherichia coli |
5.1.3.8 | AGE | - |
Synechocystis sp. PCC 6803 |
5.1.3.8 | GlcNAc 2-epimerase | - |
Synechocystis sp. PCC 6803 |
5.1.3.8 | N-acetyl-D-glucosamine 2-epimerase | - |
Synechocystis sp. PCC 6803 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.8 | 7.5 | - |
assay at | Synechocystis sp. PCC 6803 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.1.3.8 | ATP | necessary activator of the GlcNAc 2-epimerase | Synechocystis sp. PCC 6803 |