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Literature summary extracted from

  • Zhang, Y.; Tao, F.; Du, M.; Ma, C.; Qiu, J.; Gu, L.; He, X.; Xu, P.
    An efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system (2010), Appl. Microbiol. Biotechnol., 86, 481-489.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.3.3 biotechnology efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 Escherichia coli
5.1.3.8 biotechnology efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 Synechocystis sp. PCC 6803

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.3 2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production Escherichia coli
5.1.3.8 2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production Synechocystis sp. PCC 6803

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.3 33000
-
determined by SDS-PAGE Escherichia coli
5.1.3.8 40000
-
determined by SDS-PAGE Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.3 N-acetyl-D-neuraminic acid Escherichia coli
-
N-acetyl-D-mannosamine + pyruvate
-
r
5.1.3.8 N-acetyl-D-mannosamine Synechocystis sp. PCC 6803
-
N-acetyl-D-glucosamine
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.3 Escherichia coli
-
-
-
5.1.3.8 Synechocystis sp. PCC 6803
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.3 additional information
-
after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.3 N-acetyl-D-neuraminic acid
-
Escherichia coli N-acetyl-D-mannosamine + pyruvate
-
r
5.1.3.8 N-acetyl-D-mannosamine
-
Synechocystis sp. PCC 6803 N-acetyl-D-glucosamine
-
r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.3 N-acetyl-d-neuraminic acid aldolase
-
Escherichia coli
4.1.3.3 Neu5Ac aldolase
-
Escherichia coli
5.1.3.8 AGE
-
Synechocystis sp. PCC 6803
5.1.3.8 GlcNAc 2-epimerase
-
Synechocystis sp. PCC 6803
5.1.3.8 N-acetyl-D-glucosamine 2-epimerase
-
Synechocystis sp. PCC 6803

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.8 7.5
-
assay at Synechocystis sp. PCC 6803

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.8 ATP necessary activator of the GlcNAc 2-epimerase Synechocystis sp. PCC 6803