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Literature summary extracted from

  • Krogh, K.B.; Harris, P.V.; Olsen, C.L.; Johansen, K.S.; Hojer-Pedersen, J.; Borjesson, J.; Olsson, L.
    Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum (2010), Appl. Microbiol. Biotechnol., 86, 143-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expressed in Aspergillus oryzae Penicillium brasilianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 4-nitrophenyl-beta-D-glucopyranoside substrate inhibition Penicillium brasilianum
3.2.1.21 D-glucono-delta-lactone
-
 Penicillium brasilianum
3.2.1.21 D-glucose
-
 Penicillium brasilianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.09
-
 4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum
3.2.1.21 1.58
-
 cellobiose recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 115000
-
 SDS-PAGE Penicillium brasilianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Penicillium brasilianum A5A4M8
-
-
3.2.1.21 Penicillium brasilianum IBT 20888 A5A4M8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 Q Sepharose column chromatography, Superdex 75 gel filtration, Resource Q column chromatography, Source 30S column chromatography, Superdex 200 gel filtration, and phenyl Sepharose column chromatography Penicillium brasilianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Penicillium brasilianum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Penicillium brasilianum IBT 20888 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 cellobiose + H2O BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside Penicillium brasilianum 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside Penicillium brasilianum IBT 20888 2 beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bgl
-
 Penicillium brasilianum
3.2.1.21 GH3 beta-glucosidase
-
 Penicillium brasilianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 70
-
 at pH values 4.16, 4.80 and 6.17 Penicillium brasilianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 no loss in activity after 24 h of incubation at 60°C at pH 4-6 Penicillium brasilianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 43
-
 cellobiose recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum
3.2.1.21 118
-
 4-nitrophenyl-beta-D-glucopyranoside recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.8
-
-
 Penicillium brasilianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4 6 stable in the range of pH 4.0 to 6.0 while outside this pH range no residual activity is measured, at 65°C the residual activity is 50% at pH 5.0 and 6.0 (after 24 h) and 20% at pH 4.0 Penicillium brasilianum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.012
-
 D-glucono-delta-lactone recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum
3.2.1.21 0.1
-
 D-glucono-delta-lactone recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum
3.2.1.21 1.1
-
 D-glucose recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum
3.2.1.21 2.3
-
 D-glucose recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C Penicillium brasilianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Penicillium brasilianum isoelectric focusing
-
 3.9