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Literature summary extracted from

  • Cheng, L.; Mu, W.; Zhang, T.; Jiang, B.
    An L-arabinose isomerase from Acidothermus cellulolytics ATCC 43068: cloning, expression, purification, and characterization (2010), Appl. Microbiol. Biotechnol., 86, 1089-1097.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 gene araA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Acidothermus cellulolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 Cu2+ 30% inhibition at 1 mM Acidothermus cellulolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ activates and increase thermostability of the enzyme Acidothermus cellulolyticus
5.3.1.4 Mn2+ activates and increase thermostability of the enzyme Acidothermus cellulolyticus
5.3.1.4 additional information metal ions are essential for catalytic activity, but metal ions, such as Mg2+, Ni2+, and Ba2+, are poor activators, while Fe2+, Ca2+, and Zn2+ have no effect on the activity Acidothermus cellulolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 54768
-
 x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE Acidothermus cellulolyticus
5.3.1.4 55000
-
 x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE Acidothermus cellulolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Acidothermus cellulolyticus
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Acidothermus cellulolyticus A0LT86 ATCC 43068, gene araA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, ion exchange chromatography, and gel filtration Acidothermus cellulolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose conversion yield over 50% after 12 h under optimal conditions Acidothermus cellulolyticus D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Acidothermus cellulolyticus L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE Acidothermus cellulolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 75
-
-
 Acidothermus cellulolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 65
-
 purified recombinant enzyme, completely stable for 2 h Acidothermus cellulolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7.5
-
-
 Acidothermus cellulolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.4 6 7.5 over 90% of maximal activity at pH 6.0, maximal activity at pH 7.5 Acidothermus cellulolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 6
-
 25°C, purified recombinant enzyme, 80% activity remaining after 12 h, 70% after 24 h Acidothermus cellulolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.4 Acidothermus cellulolyticus sequence calculation
-
 5.63