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Literature summary extracted from

  • Zhang, J.D.; Li, A.T.; Yang, Y.; Xu, J.H.
    Sequence analysis and heterologous expression of a new cytochrome P450 monooxygenase from Rhodococcus sp. for asymmetric sulfoxidation (2010), Appl. Microbiol. Biotechnol., 85, 615-624.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.1 biotechnology enzymatic activity of P450SMO makes it an attractive biocatalyst for asymmetric synthesis of enantiopure sulfoxides Rhodococcus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 into the pET28a (+) vector and expressed in Escherichia coli BL21 (DE3) Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.1 imidazole at 20 mM, only half of P450SMO activity remains Rhodococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.1 Fe2+ the C-terminal reductase portion of P450SMO comprises a [2Fe2S] ferredoxin center Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.1 87190
-
 sequence analysis Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Rhodococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.1 gel filtration Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 7-ethoxycoumarin + NADPH + H+ + O2 mediates the O-dealkylation Rhodococcus sp. 7-hydroxycoumarin + NADP+ + H2O + ?
-
 ?
1.14.14.1 additional information no hydroxylation activity towards naphthalene, indene, ethyl benzene, and m-xylene Rhodococcus sp. ?
-
 ?
1.14.14.1 p-chlorothioanisole + [reduced NADPH-hemoprotein reductase] + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.1 p-fluorothioanisole + [reduced NADPH-hemoprotein reductase] + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.1 p-methoxythioanisole + [reduced NADPH-hemoprotein reductase] + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.1 p-tolyl methyl sulfide + [reduced NADPH-hemoprotein reductase] + O2
-
 Rhodococcus sp. ?
-
 ?
1.14.14.1 phenyl ethyl sulfide + [reduced NADPH-hemoprotein reductase] + O2
-
 Rhodococcus sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 cytochrome P450 monooxygenase
-
 Rhodococcus sp.
1.14.14.1 P450SMO
-
 Rhodococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.1 30
-
 toward p-chlorothioanisole Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.1 7
-
 toward p-chlorothioanisole Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.1 FMN the C-terminal reductase portion of P450SMO comprises an FMN-binding Rhodococcus sp.
1.14.14.1 NADH the C-terminal reductase portion of P450SMO comprises an NADH-binding Rhodococcus sp.
1.14.14.1 NADPH
-
 Rhodococcus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.1 Rhodococcus sp.
-
-
 5.17