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Literature summary extracted from

  • Singh, B.; Lee, C.B.; Sohng, J.K.
    Precursor for biosynthesis of sugar moiety of doxorubicin depends on rhamnose biosynthetic pathway in Streptomyces peucetius ATCC 27952 (2010), Appl. Microbiol. Biotechnol., 85, 1565-1574.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.46 PCR product cloned into pET-32a(+) to construct pDH32 and transformed into Escherichia coli BL21 (DE3) to give pDH32BL for protein expression. PCR product cloned into EcoRI/HindIII of pIBR25 to construct a recombinant vector, pDHC25. The vector pDHC25 subsequently transformed into the rmbB-knockout mutant of Streptomyces peucetius DHNEO by PEG-mediated protoplast transformation Streptomyces peucetius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.46 Mg2+
-
 Streptomyces peucetius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.46 36500
-
 sequence analysis Streptomyces peucetius
4.2.1.46 54000
-
 recombinant enzyme Streptomyces peucetius

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Streptomyces peucetius C5J046 ATCC 27952
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.46 by cobalt-immobilized affinity chromatography Streptomyces peucetius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-glucose enzyme consists of a C-terminal sugar-nucleotide binding domain and an N-terminal NAD+ cofactor-binding domain Streptomyces peucetius dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.46 RmbB
-
 Streptomyces peucetius
4.2.1.46 TDP-D-glucose 4,6-dehydratase
-
 Streptomyces peucetius

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.46 NAD+ enzyme consists of an N-terminal NAD+ cofactor-binding domain and a C-terminal sugar-nucleotide binding domain Streptomyces peucetius

General Information

EC Number General Information Comment Organism
4.2.1.46 malfunction inactivation of rmbB abolishes the production of doxorubicin while complementation of the same gene in an rmbB knockout mutant restores the doxorubicin production Streptomyces peucetius
4.2.1.46 physiological function rmbB provides dTDP-4-dehydro-6-deoxy-D-glucose as a nucleotide sugar precursor for the biosynthesis of doxorubicin Streptomyces peucetius