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Literature summary extracted from

  • Rzeznicka, K.; Schaetzle, S.; Boettcher, D.; Klein, J.; Bornscheuer, U.T.
    Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterisation (2010), Appl. Microbiol. Biotechnol., 85, 1417-1425.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 expressed in Escherichia coli BL21(DE3) cells Rhodococcus equi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe3+ a Fe-type NHase Rhodococcus equi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 23000
-
 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi
4.2.1.84 24000
-
 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus equi
-
-
-
4.2.1.84 Rhodococcus equi TG328-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 Sepharose 6 column chromatography Rhodococcus equi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 844
-
 crude cell extract towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) Rhodococcus equi
4.2.1.84 6290
-
 after 7.5fold purification towards methacrylonitrile, in 50 mM Tris-HCl (pH 7.5) Rhodococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 (R,S)-2-(4-nitrophenyl)-propionitrile + H2O 39% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 (R,S)-2-bromopropionitrile + H2O 47% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 (R,S)-2-chloropropionitrile + H2O 48% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 (R,S)-2-phenylbutyronitrile + H2O 51% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 (R,S)-2-phenylpropionitrile + H2O 43% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 (R,S)-3-oxo-2-phenylbutyronitrile + H2O 43% conversion Rhodococcus equi ?
-
 ?
4.2.1.84 2-phenylacetonitrile + H2O
-
 Rhodococcus equi 2-phenylpropionamide
-
 ?
4.2.1.84 3-cyanopyridine + H2O
-
 Rhodococcus equi pyridine-3-carbamide
-
 ?
4.2.1.84 benzonitrile + H2O
-
 Rhodococcus equi benzoic acid amide
-
 ?
4.2.1.84 benzonitrile + H2O
-
 Rhodococcus equi TG328-2 benzoic acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O
-
 Rhodococcus equi isobutyric acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O
-
 Rhodococcus equi TG328-2 isobutyric acid amide
-
 ?
4.2.1.84 methacrylonitrile + H2O
-
 Rhodococcus equi methacrylamide
-
 ?
4.2.1.84 additional information besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially Rhodococcus equi ?
-
 ?
4.2.1.84 additional information no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid Rhodococcus equi ?
-
 ?
4.2.1.84 additional information besides aromatic and heterocyclic nitriles, aliphatic ones are hydrated preferentially Rhodococcus equi TG328-2 ?
-
 ?
4.2.1.84 additional information no activity with (R,S)-2-(4-isobutylphenyl)propionitrile and (R,S)-3-(1-cyanoethyl)benzoic acid Rhodococcus equi TG328-2 ?
-
 ?
4.2.1.84 valeronitrile + H2O almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile Rhodococcus equi n-pentanoic acid amide
-
 ?
4.2.1.84 valeronitrile + H2O almost 9000fold higher activity towards valeronitrile compared to (R,S)-2-phenylpropionitrile Rhodococcus equi TG328-2 n-pentanoic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 heterodimer 1 * 24000 + 1 * 23000, SDS-PAGE Rhodococcus equi

Synonyms

EC Number Synonyms Comment Organism
4.2.1.84 NHase
-
 Rhodococcus equi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 30
-
-
 Rhodococcus equi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 4 70 highest stability at 4°C in thermal inactivation studies between 4°C and 50°C, the enzyme is inactivated immediately at 70°C Rhodococcus equi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 6 8.5
-
 Rhodococcus equi

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.84 pyrroloquinoline quinone
-
 Rhodococcus equi