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Literature summary extracted from

  • Qu, Y.; Spain, J.C.
    Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. strain JS329 (2010), Appl. Environ. Microbiol., 76, 1417-1422.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.8 expression in Escherichia coli Bradyrhizobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.64 2,2'-dipyridyl 1 mM, 98% loss of activity Bradyrhizobium sp.
1.13.11.64 o-phenanthroline 1 mM, complete loss of activity Bradyrhizobium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.64 Fe2+ ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions Bradyrhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.8 5-nitroanthranilate + H2O Bradyrhizobium sp. initial step in biodegradation of 5-nitroanthranilic acid as sole carbon, nitrogen and energy source 5-nitrosalicylate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.64 Bradyrhizobium sp. D3WZ86
-
-
3.5.99.8 Bradyrhizobium sp. D3WZ85
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.13.11.64 ultrafiltration abolishes the enzymatic activity against 5-nitrosalicylate, which is restored by addition of ferrous but not ferric ions Bradyrhizobium sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.64 -20°C, 40% to 50% loss of original activity after 1 week of storage Bradyrhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.64 4-nitro-6-oxohepta-2,4-dienedioate
-
 Bradyrhizobium sp. 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite 2nd step of reaction, spontaneous ?
1.13.11.64 5-nitrosalicylate + O2 oxidation catalyzed by cell extracts requires 0.97 mol O2/mol 5-nitrosalicylate Bradyrhizobium sp. 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite overall reaction ?
1.13.11.64 5-nitrosalicylate + O2
-
 Bradyrhizobium sp. 4-nitro-6-oxohepta-2,4-dienedioate 1st step of reaction ?
1.13.11.64 salicylate + O2
-
 Bradyrhizobium sp. ?
-
 ?
3.5.99.8 5-nitroanthranilate + H2O
-
 Bradyrhizobium sp. 5-nitrosalicylate + NH3
-
 ?
3.5.99.8 5-nitroanthranilate + H2O initial step in biodegradation of 5-nitroanthranilic acid as sole carbon, nitrogen and energy source Bradyrhizobium sp. 5-nitrosalicylate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.64 5NSA dioxygenase
-
 Bradyrhizobium sp.
1.13.11.64 naaB
-
 Bradyrhizobium sp.
3.5.99.8 5NAA deaminase
-
 Bradyrhizobium sp.
3.5.99.8 NAAA
-
 Bradyrhizobium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.64 additional information no requirement of NADPH Bradyrhizobium sp.
3.5.99.8 additional information no added cofactors are required for the transformation Bradyrhizobium sp.