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Literature summary extracted from

  • Placier, G.; Watzlawick, H.; Rabiller, C.; Mattes, R.
    Evolved beta-galactosidases from Geobacillus stearothermophilus with improved transgalactosylation yield for galacto-oligosaccharide production (2009), Appl. Environ. Microbiol., 75, 6312-6321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in Escherichia coli JM109 cells Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.23 R109K the mutant shows decreased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased Geobacillus stearothermophilus
3.2.1.23 R109V the mutant shows increased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased Geobacillus stearothermophilus
3.2.1.23 R109W the mutant shows increased specific activity towards 4-nitrophenyl-beta-D-galactopyranoside compared to the wild type enzyme, the apparent Km values toward the natural substrates lactose and lactosucrose are strongly increased Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 1.78
-
 lactose wild type enzyme, at 37°C Geobacillus stearothermophilus
3.2.1.23 5
-
 lactosucrose wild type enzyme, at 37°C Geobacillus stearothermophilus
3.2.1.23 84
-
 lactose mutant enzyme R109K, at 37°C Geobacillus stearothermophilus
3.2.1.23 100
-
 lactose mutant enzyme R109V, at 37°C Geobacillus stearothermophilus
3.2.1.23 114
-
 lactose mutant enzyme R109W, at 37°C Geobacillus stearothermophilus
3.2.1.23 136
-
 lactosucrose mutant enzyme R109K, at 37°C Geobacillus stearothermophilus
3.2.1.23 142
-
 lactosucrose mutant enzyme R109V, at 37°C Geobacillus stearothermophilus
3.2.1.23 160
-
 lactosucrose mutant enzyme R109W, at 37°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 78000
-
 SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Geobacillus stearothermophilus
-
-
-
3.2.1.23 Geobacillus stearothermophilus KVE39
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.19
-
 wild type enzyme, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 0.22
-
 mutant enzyme R109K, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 0.47
-
 mutant enzyme R109K, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 0.5
-
 wild type enzyme, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 0.6
-
 mutant enzyme R109W, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 1.52
-
 mutant enzyme R109V, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 1.79
-
 mutant enzyme R109W, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus
3.2.1.23 4.9
-
 mutant enzyme R109V, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus KVE39 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Geobacillus stearothermophilus D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Geobacillus stearothermophilus KVE39 D-galactose + D-glucose
-
 ?
3.2.1.23 lactosucrose + H2O
-
 Geobacillus stearothermophilus D-galactose + ?
-
 ?
3.2.1.23 lactosucrose + H2O
-
 Geobacillus stearothermophilus KVE39 D-galactose + ?
-
 ?
3.2.1.23 additional information during the transglycosylation reaction with lactose of the evolved beta-galactosidases, a major trisaccharide, beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranosyl-(1-4)-D-glucopyranoside (3'-galactosyl-lactose) is formed. At the lactose concentration of 18% (w/v), this trisaccharide is obtained in yields of 11.5% (w/w) with BgaB mutant enzyme R109K, 21% (w/w) with BgaB mutant enzyme R109V, 23% (w/w) with BgaB mutant enzyme R109W, and 2% (w/w) with the wild type BgaB enzyme Geobacillus stearothermophilus ?
-
 ?
3.2.1.23 additional information during the transglycosylation reaction with lactose of the evolved beta-galactosidases, a major trisaccharide, beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranosyl-(1-4)-D-glucopyranoside (3'-galactosyl-lactose) is formed. At the lactose concentration of 18% (w/v), this trisaccharide is obtained in yields of 11.5% (w/w) with BgaB mutant enzyme R109K, 21% (w/w) with BgaB mutant enzyme R109V, 23% (w/w) with BgaB mutant enzyme R109W, and 2% (w/w) with the wild type BgaB enzyme Geobacillus stearothermophilus KVE39 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 BgaB
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 65
-
-
 Geobacillus stearothermophilus