Literature summary extracted from
Chen, I.; Lin, W.; Hsu, S.; Thiruvengadam, V.; Hsu, W.
Isolation and characterization of a novel lysine racemase from a soil metagenomic library (2009), Appl. Environ. Microbiol., 75, 5161-5166.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.1.1.5 |
expression in Escherichia coli |
uncultured bacterium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.1.1.5 |
dipicolinic acid |
complete inhibition by metal-chelating agents in 50 mM Tris-HCl buffer (pH 8.0) at 30°C |
uncultured bacterium |
|
5.1.1.5 |
EDTA |
complete inhibition by metal-chelating agents in 50 mM Tris-HCl buffer (pH 8.0) at 30°C |
uncultured bacterium |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.1.1.5 |
16.21 |
- |
L-lysine |
in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
|
5.1.1.5 |
23.48 |
- |
D-Lysine |
in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.1.1.5 |
Co2+ |
addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 114.5% |
uncultured bacterium |
|
5.1.1.5 |
Mg2+ |
addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 83.6 |
uncultured bacterium |
|
5.1.1.5 |
Mn2+ |
addition of 2 mM extrinsic divalent ions enhances the enzyme activity up to 97.3% |
uncultured bacterium |
|
5.1.1.5 |
Zn2+ |
analyzing of bound metal ions in purified Lyr with an inductively coupled plasma spectrometer shows that dialyzed Lyr contains 0.77 mol of Zn2+ per mol of the dimeric enzyme, while cobalt, magnesium, manganese, nickel, or copper ions are absent |
uncultured bacterium |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.1.1.5 |
42700 |
- |
protein composed of 393 amino acids with the calculated molecular mass, confirmed by SDS-PAGE |
uncultured bacterium |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.1.1.5 |
L-lysine |
uncultured bacterium |
- |
D-lysine |
enzyme exhibits higher specific activity toward lysine in the L-lysine to D-lysine direction than in the reverse reaction |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.1.5 |
uncultured bacterium |
C7ACH5 |
from garden soil, the lyr gene may have only recently evolved from Escherichia coli N-acetyl-gamma-glutamyl-phosphate reductase and Deinococcus radiodurans N-acyl amino acid racemase, the partial regions of both enzymes might have been combined to evolve a novel enzyme, in which the new catalytic sites are created for catalyzing the racemization of lysine |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.1.1.5 |
by nickel chelate affinity chromatography |
uncultured bacterium |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.1.1.5 |
1.68 |
- |
substrate 10 mM D-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
5.1.1.5 |
3.61 |
- |
substrate 10 mM L-lysine, in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.1.1.5 |
L-lysine |
- |
uncultured bacterium |
D-lysine |
enzyme exhibits higher specific activity toward lysine in the L-lysine to D-lysine direction than in the reverse reaction |
r |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.1.1.5 |
lyr |
- |
uncultured bacterium |
5.1.1.5 |
lysine racemase |
- |
uncultured bacterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.1.1.5 |
30 |
- |
maximum activity |
uncultured bacterium |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
5.1.1.5 |
50 |
- |
the enzyme shows considerable stability at 50°C, with a half-life of about 3 days |
uncultured bacterium |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
5.1.1.5 |
2.16 |
- |
D-Lysine |
in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
|
5.1.1.5 |
5.1 |
- |
L-lysine |
in 50 mM Tris-HCl (pH 8.0), 2 mM cobalt chloride at 30°C |
uncultured bacterium |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.1.1.5 |
8 |
- |
50 mM Tris-HCl |
uncultured bacterium |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
5.1.1.5 |
5 |
9 |
about 15% of maximal activity is observed at pHs of below 5 and above 9 |
uncultured bacterium |