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Literature summary extracted from

  • Feng, X.; Mouttaki, H.; Lin, L.; Huang, R.; Wu, B.; Hemme, C.L.; He, Z.; Zhang, B.; Hicks, L.M.; Xu, J.; Zhou, J.; Tang, Y.J.
    Characterization of the central metabolic pathways in Thermoanaerobacter sp. strain X514 via isotopomer-assisted metabolite analysis (2009), Appl. Environ. Microbiol., 75, 5001-5008.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.3.21 biotechnology fermentation of C5 and C6 sugars to ethanols and other metabolites under thermophilic conditions Thermoanaerobacter sp. X514

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.21 pyruvate + acetyl-CoA + H2O Thermoanaerobacter sp. X514
-
 (2R)-2-hydroxy-2-methylbutanedioate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.21 Thermoanaerobacter sp. B0K6M2
-
-
2.3.3.21 Thermoanaerobacter sp. X514 B0K6M2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.21 cells are centrifuged, pellet sonicated, resuspended in 0.1 M N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer, pH 7.5 Thermoanaerobacter sp. X514

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.21 0.027
-
 production of CoA measured at 60°C, pH 7.5 Thermoanaerobacter sp. X514

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O the organism exhibits an inactive pentose phosphate pathway and an alternate isoleucine biosynthesis pathway via the citramalate pathway that uses pyruvate and acetyl-CoA as precursors Thermoanaerobacter sp. CoA + (R)-citramalate
-
 ?
2.3.3.21 pyruvate + acetyl-CoA + H2O
-
 Thermoanaerobacter sp. X514 (2R)-2-hydroxy-2-methylbutanedioate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.21 CimA
-
 Thermoanaerobacter sp.
2.3.3.21 cimA protein
-
 Thermoanaerobacter sp. X514
2.3.3.21 citramalate synthase
-
 Thermoanaerobacter sp.
2.3.3.21 citramalate synthase
-
 Thermoanaerobacter sp. X514

General Information

EC Number General Information Comment Organism
2.3.3.21 metabolism biosynthesis of isoleucine from the enzyme product citramalate ((2R)-2-hydroxy-2-methylbutanedioate) Thermoanaerobacter sp. X514
2.3.3.21 physiological function the organism exhibits an inactive pentose phosphate pathway and an alternate isoleucine biosynthesis pathway via the citramalate pathway that uses pyruvate and acetyl-CoA as precursors Thermoanaerobacter sp.