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Literature summary extracted from

  • Hanniffy, S.B.; Philo, M.; Pelaez, C.; Gasson, M.J.; Requena, T.; Martinez-Cuesta, M.C.
    Heterologous production of methionine-gamma-lyase from Brevibacterium linens in Lactococcus lactis and formation of volatile sulfur compounds (2009), Appl. Environ. Microbiol., 75, 2326-2332.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.4.1.11 nutrition importance in flavor formation during cheese ripening Brevibacterium linens BL2

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.11 expression in Lactococcus lactis Brevibacterium linens BL2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 170000
-
 SDS-PAGE Brevibacterium linens BL2

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Brevibacterium linens BL2 Q5MQ31
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11 affinity chromatography on nickel-nitrilotriacetic acid resin Brevibacterium linens BL2

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 additional information
-
 recombinant enzyme, substrate L-cystathione, with nisin induction, value from 15 to 65 micromol/min/mg Brevibacterium linens BL2
4.4.1.11 additional information
-
 recombinant enzyme, substrate L-cystine, with nisin induction, value from 21 to 137 micromol/min/mg Brevibacterium linens BL2
4.4.1.11 additional information
-
 recombinant enzyme, substrate L-methionine, with nisin induction, value from 21 to 103 micromol/min/mg Brevibacterium linens BL2
4.4.1.11 14
-
 recombinant enzyme, substrate L-cystathionine, without nisin induction Brevibacterium linens BL2
4.4.1.11 19
-
 recombinant enzyme, substrate L-cystine or L-methionine, without nisin induction Brevibacterium linens BL2
4.4.1.11 60
-
 wild-type enzyme, substrate L-cystathionine, without nisin induction Brevibacterium linens BL2
4.4.1.11 90
-
 wild-type enzyme, substrate L-methionine, without nisin induction Brevibacterium linens BL2
4.4.1.11 123
-
 wild-type enzyme, substrate L-cystine, without nisin induction Brevibacterium linens BL2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 L-cystathionine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
4.4.1.11 L-cysteine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
4.4.1.11 L-cystine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?
4.4.1.11 L-methionine + H2O assay at pH 8.0, 37°C Brevibacterium linens BL2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.11 methionine-gamma-lyase
-
 Brevibacterium linens BL2

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.11 37
-
 assay at Brevibacterium linens BL2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.11 8
-
 assay at Brevibacterium linens BL2