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Literature summary extracted from

  • Menendez, C.; Banguela, A.; Caballero-Mellado, J.; Hernandez, L.
    Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus (2009), Appl. Environ. Microbiol., 75, 1782-1785.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 gene lsdA, organized in an operon with exolevanase LsdB, transcriptional linkage of the chromosomal lsdAB gene cluster Gluconacetobacter diazotrophicus
3.2.1.65 gene lsdB, organized in one operon with gene lsdA, encoding levansucrase, organization of the lsdAB operon and relevant features of the intergenic sequence, expression analysis, overview Gluconacetobacter diazotrophicus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 additional information construction of mutants AD6, AD8, and AD9, that are unable to secrete LsdA due to nptII insertions in the type II secretion, gsp, operon. None of the mutants released extracellular LsdB, the exolavanase organized in one operon with the levansucrase LsdA Gluconacetobacter diazotrophicus
3.2.1.65 additional information type II secretion mutants AD6, AD8, and AD9, unable to secrete LsdA due to nptII insertions in the type II secretion, the mutants fail to transfer LsdB across the outer membrane, impeding levan hydrolysis Gluconacetobacter diazotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 extracellular secretion of LsdA Gluconacetobacter diazotrophicus
-
-
3.2.1.65 extracellular
-
 Gluconacetobacter diazotrophicus
-
-
3.2.1.65 additional information mature LsdB is unlikely to attach cell membranes Gluconacetobacter diazotrophicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Gluconacetobacter diazotrophicus Q43998 gene lsdA and lsdB
-
3.2.1.65 Gluconacetobacter diazotrophicus
-
 gene lsdB
-
3.2.1.65 Gluconacetobacter diazotrophicus SRT4
-
 gene lsdB
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.65 additional information enzyme expression analysis under different growth conditions, overview Gluconacetobacter diazotrophicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 0.25
-
 intracellular wild-type enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.33
-
 extracellular mutant AD6 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.52
-
 extracellular mutant AD8 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.69
-
 intracellular mutant AD9 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.71
-
 extracellular mutant AD9 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.73
-
 intracellular mutant AD8 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 0.75
-
 intracellular mutant AD6 enzyme Gluconacetobacter diazotrophicus
3.2.1.65 14.24
-
 extracellular wild-type enzyme Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan + H2O substrate from Erwinia herbicola Gluconacetobacter diazotrophicus ?
-
 ?
3.2.1.65 levan + H2O substrate from Erwinia herbicola Gluconacetobacter diazotrophicus SRT4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 LsdA
-
 Gluconacetobacter diazotrophicus
3.2.1.65 exolevanase
-
 Gluconacetobacter diazotrophicus
3.2.1.65 LsdB
-
 Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 30
-
 assay at Gluconacetobacter diazotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 5
-
 assay at Gluconacetobacter diazotrophicus

Expression

EC Number Organism Comment Expression
3.2.1.65 Gluconacetobacter diazotrophicus lsdB transcription is repressed by D-glucose down
3.2.1.65 Gluconacetobacter diazotrophicus lsdB transcription is induced during growth with low fructose concentrations of 0.44 to 33 mM up