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Literature summary extracted from

  • Nakajima, M.; Kitaoka, M.
    Identification of lacto-N-biose I phosphorylase from Vibrio vulnificus CMCP6 (2008), Appl. Environ. Microbiol., 74, 6333-6337.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.211 0.064
-
phosphate with beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus
2.4.1.211 1.3
-
phosphate with beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus
2.4.1.211 3.6
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus
2.4.1.211 10
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.211 83580
-
calculated from amino acid sequence Vibrio vulnificus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.211 Vibrio vulnificus A0A3Q0KZS1
-
-
2.4.1.211 Vibrio vulnificus CMCP6LNBP A0A3Q0KZS1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose Vibrio vulnificus alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose Vibrio vulnificus CMCP6LNBP alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine Vibrio vulnificus alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
2.4.1.211 beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine Vibrio vulnificus CMCP6LNBP alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
2.4.1.211 additional information no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose Vibrio vulnificus ?
-
?
2.4.1.211 additional information no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose Vibrio vulnificus CMCP6LNBP ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.211 beta-1,3-galactosyl-N-acetylhexosamine phosphorylase
-
Vibrio vulnificus
2.4.1.211 Gal-GlyNAcP
-
Vibrio vulnificus
2.4.1.211 lacto-N-biose I phosphorylase
-
Vibrio vulnificus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.211 20
-
-
Vibrio vulnificus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.211 30 55 the enzyme is stable at temperatures up to 30°C during incubation for 30 min, the reactions proceed linearly for 5 min at temperatures up to 37°C but do not proceed linearly at 45°C, no reaction is observed at 55°C Vibrio vulnificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.211 2.2
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus
2.4.1.211 45
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.211 6.5 7.5
-
Vibrio vulnificus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.211 6.5 9
-
Vibrio vulnificus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.211 0.22
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus
2.4.1.211 13
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C Vibrio vulnificus