EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.211 | 0.064 | - |
phosphate | with beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus | |
2.4.1.211 | 1.3 | - |
phosphate | with beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as cosubstrate, in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus | |
2.4.1.211 | 3.6 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus | |
2.4.1.211 | 10 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 83580 | - |
calculated from amino acid sequence | Vibrio vulnificus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.211 | Vibrio vulnificus | A0A3Q0KZS1 | - |
- |
2.4.1.211 | Vibrio vulnificus CMCP6LNBP | A0A3Q0KZS1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose | Vibrio vulnificus | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine + phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine is galacto-N-biose | Vibrio vulnificus CMCP6LNBP | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | Vibrio vulnificus | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
2.4.1.211 | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate | beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine is lacto-N-biose I, best substrate being 7times more effective than beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | Vibrio vulnificus CMCP6LNBP | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
2.4.1.211 | additional information | no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose | Vibrio vulnificus | ? | - |
? | |
2.4.1.211 | additional information | no activity with D-glucose, D-galactose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose | Vibrio vulnificus CMCP6LNBP | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.211 | beta-1,3-galactosyl-N-acetylhexosamine phosphorylase | - |
Vibrio vulnificus |
2.4.1.211 | Gal-GlyNAcP | - |
Vibrio vulnificus |
2.4.1.211 | lacto-N-biose I phosphorylase | - |
Vibrio vulnificus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 20 | - |
- |
Vibrio vulnificus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 30 | 55 | the enzyme is stable at temperatures up to 30°C during incubation for 30 min, the reactions proceed linearly for 5 min at temperatures up to 37°C but do not proceed linearly at 45°C, no reaction is observed at 55°C | Vibrio vulnificus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.211 | 2.2 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus | |
2.4.1.211 | 45 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 6.5 | 7.5 | - |
Vibrio vulnificus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 6.5 | 9 | - |
Vibrio vulnificus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.211 | 0.22 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus | |
2.4.1.211 | 13 | - |
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine | in 50 mM Tris-HCl (pH 7.5), 10 mM sodium-potassium phosphate buffer, at 30°C | Vibrio vulnificus |