EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.9.1 | biotechnology | co-immobilization of enzyme with redox polymer poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A per m2 depending on the substrate and the operation temperature. The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the (S)-2-alcohols, the enantioselectivity increases with increasing chain length. The enantiomeric ratio E increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol | Comamonas testosteroni |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.9.1 | 0.005 | - |
1-butanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.005 | - |
1-Pentanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.005 | - |
1-Octanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.006 | - |
1-Pentanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.006 | - |
1-Octanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.006 | - |
1-butanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.04 | - |
(S)-2-heptanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.05 | - |
2-Octanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.05 | - |
(S)-2-octanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.05 | - |
2-Octanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.05 | - |
(S)-2-octanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.06 | - |
1-propanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.06 | - |
(S)-2-heptanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.06 | - |
1-propanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.09 | - |
(S)-2-hexanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.09 | - |
(S)-2-hexanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.1 | - |
(R)-solketal | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.11 | - |
(R)-solketal | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.24 | - |
5-methyl-2-hexanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.3 | - |
1,3-butanediol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.4 | - |
2-Pentanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.4 | - |
(R)-2-octanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.4 | - |
4-decanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.4 | - |
4-decanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.6 | - |
(R)-2-hexanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.6 | - |
2-Pentanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.7 | - |
4-heptanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.7 | - |
(R)-2-heptanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.7 | - |
4-heptanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.75 | - |
4-methyl-2-pentanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.8 | - |
Cyclohexanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.8 | - |
3-nonanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.8 | - |
3-nonanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.83 | - |
Cyclohexanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.9 | - |
3-octanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.9 | - |
3-methyl-2-pentanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.9 | - |
3-octanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 0.99 | - |
(S)-solketal | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 1 | - |
(S)-solketal | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 1.1 | - |
(R)-2-heptanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 1.2 | - |
(R)-2-hexanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 2.2 | - |
ethanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 2.2 | - |
ethanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 2.9 | - |
3-Heptanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 4.3 | - |
3-Heptanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 4.5 | - |
(S)-glycidol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 4.5 | - |
(S)-2-butanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 4.8 | - |
(R)-glycidol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 5 | - |
(S)-2-butanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 5.3 | - |
3-Hexanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 6.3 | - |
3-Hexanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 8 | - |
2-butanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 12 | - |
2-butanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 22 | - |
2-propanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 28 | - |
3-Pentanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 28 | - |
(R)-2-butanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 30 | - |
(R)-2-butanol | enzyme electrode, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 30 | - |
2-propanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni | |
1.1.9.1 | 30 | - |
3-Pentanol | soluble enzyme, pH 7.7, presence of CaCl2 | Comamonas testosteroni |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.9.1 | Comamonas testosteroni | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.9.1 | (R)-2-butanol + ferricyanide | enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively | Comamonas testosteroni | butan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | (R)-2-heptanol + ferricyanide | enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively | Comamonas testosteroni | heptan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | (R)-2-hexanol + ferrocyanide | enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively | Comamonas testosteroni | hexan-2-one + ferricyanide | - |
? | |
1.1.9.1 | (R)-2-octanol + ferrocyanide | enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively | Comamonas testosteroni | octan-2-one + ferricyanide | - |
? | |
1.1.9.1 | (R)-glycidol + ferrocyanide | enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction | Comamonas testosteroni | ? + ferricyanide | - |
? | |
1.1.9.1 | (R)-solketal + ferrocyanide | enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively | Comamonas testosteroni | ? + ferricyanide | - |
? | |
1.1.9.1 | (S)-2-butanol + ferricyanide | enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively | Comamonas testosteroni | butan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | (S)-2-heptanol + ferricyanide | enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively | Comamonas testosteroni | heptan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | (S)-2-hexanol + ferrocyanide | enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively | Comamonas testosteroni | hexan-2-one + ferricyanide | - |
? | |
1.1.9.1 | (S)-2-octanol + ferrocyanide | enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively | Comamonas testosteroni | octan-2-one + ferricyanide | - |
? | |
1.1.9.1 | (S)-glycidol + ferrocyanide | enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction | Comamonas testosteroni | ? + ferricyanide | - |
? | |
1.1.9.1 | (S)-solketal + ferrocyanide | enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively | Comamonas testosteroni | ? + ferricyanide | - |
? | |
1.1.9.1 | 1,3-butanediol + ferricyanide | enzyme electrode, 91% of vmax with 1-butanol | Comamonas testosteroni | ? + ferrocyanide | - |
? | |
1.1.9.1 | 1-butanol + ferricyanide | maximum velocity rate for both enzyme electrode and soluble enzyme | Comamonas testosteroni | butanal + ferrocyanide | - |
? | |
1.1.9.1 | 1-octanol + ferrocyanide | enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol | Comamonas testosteroni | octanal + ferricyanide | - |
? | |
1.1.9.1 | 1-pentanol + ferrocyanide | enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol | Comamonas testosteroni | pentanal + ferricyanide | - |
? | |
1.1.9.1 | 1-propanol + ferricyanide | enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 90% of vmax with 1-butanol | Comamonas testosteroni | propanal + ferrocyanide | - |
? | |
1.1.9.1 | 2-butanol + ferrocyanide | enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol | Comamonas testosteroni | butanone + ferricyanide | - |
? | |
1.1.9.1 | 2-octanol + ferrocyanide | enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol | Comamonas testosteroni | octan-2-one + ferricyanide | - |
? | |
1.1.9.1 | 2-pentanol + ferrocyanide | enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol | Comamonas testosteroni | pentan-2-one + ferricyanide | - |
? | |
1.1.9.1 | 2-propanol + ferrocyanide | enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol | Comamonas testosteroni | acetone + ferricyanide | - |
? | |
1.1.9.1 | 3-heptanol + ferrocyanide | enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol | Comamonas testosteroni | heptan-3-one + ferricyanide | - |
? | |
1.1.9.1 | 3-hexanol + ferrocyanide | enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol | Comamonas testosteroni | hexan-3-one + ferricyanide | - |
? | |
1.1.9.1 | 3-methyl-2-pentanol + ferricyanide | enzyme electrode, 3.7% of vmax with 1-butanol | Comamonas testosteroni | 3-methylpentan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 3-methyl-2-pentanol + ferricyanide | enzyme electrode, 9.3% of vmax with 1-butanol | Comamonas testosteroni | 3-methylpentan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 3-nonanol + ferricyanide | enzyme electrode, 4.7% of vmax with 1-butanol, soluble enzyme, 4.5% of vmax with 1-butanol | Comamonas testosteroni | nonan-3-one + ferrocyanide | - |
? | |
1.1.9.1 | 3-octanol + ferrocyanide | enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol | Comamonas testosteroni | octan-3-one + ferricyanide | - |
? | |
1.1.9.1 | 3-pentanol + ferrocyanide | enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol | Comamonas testosteroni | pentan-3-one + ferricyanide | - |
? | |
1.1.9.1 | 4-decanol + ferricyanide | enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 3.6% of vmax with 1-butanol | Comamonas testosteroni | decan-4-one + ferrocyanide | - |
? | |
1.1.9.1 | 4-heptanol + ferricyanide | enzyme electrode, 9.3% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol | Comamonas testosteroni | heptan-4-one + ferrocyanide | - |
? | |
1.1.9.1 | 4-methyl-2-pentanol + ferricyanide | enzyme electrode, 5.6% of vmax with 1-butanol | Comamonas testosteroni | 4-methylpentan-2-one + ferrocyanide | - |
? | |
1.1.9.1 | 5-methyl-2-hexanol + ferricyanide | - |
Comamonas testosteroni | 5-methylhexan-2-one + ferrocyanide | - |
r | |
1.1.9.1 | cyclohexanol + ferricyanide | enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol | Comamonas testosteroni | cyclohexanone + ferrocyanide | - |
? | |
1.1.9.1 | ethanol + ferricyanide | 86% of vmax with 1-butanol, soluble enzyme, 75% of vmax with 1-butanol | Comamonas testosteroni | ethanal + ferrocyanide | - |
? | |
1.1.9.1 | additional information | no substrate: D-glucose, D-galactose, D-fructose, Alpha-D-methylglucoside, mannitol | Comamonas testosteroni | ? | - |
? | |
1.1.9.1 | additional information | The affinity of the enzyme for aliphatic alcohols increases with the chain length of the substrate. The same property is observed for secondary alcohols in the series 2-propanol to 2-octanol | Comamonas testosteroni | ? | - |
? |