BRENDA - Enzyme Database show

Engineering Escherichia coli for fermentative dihydrogen production: potential role of NADH-ferredoxin oxidoreductase from the hydrogenosome of anaerobic protozoa

Do, P.M.; Angerhofer, A.; Hrdy, I.; Bardonova, L.; Ingram, L.O.; Shanmugam, K.T.; Appl. Biochem. Biotechnol. 153, 21-33 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.6.99.3
expressed in Escherichia coli, active protein as well as individual subunits are expressed separately
Trichomonas vaginalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.99.3
0.06
-
ferricyanide
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.1
-
NADH
electron acceptor benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.17
-
benzyl viologen
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.22
-
NADH
electron acceptor methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.31
-
NADH
electron acceptor ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.44
-
methyl viologen
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.99.3
24000
-
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
1.6.99.3
51000
-
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
1.6.99.3
69100
-
gel filtration
Trichomonas vaginalis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.6.99.3
Trichomonas vaginalis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.6.99.3
-
Trichomonas vaginalis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.6.99.3
24
-
substrate Clostridium acetobutylicum ferredoxin, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
392
-
substrate methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
582
-
substrate benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
604
-
substrate ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.99.3
2 ferricyanide + NADH
-
701758
Trichomonas vaginalis
2 ferrocyanide + NAD+ + H+
-
-
-
?
1.6.99.3
NADH + benzyl viologen
-
701758
Trichomonas vaginalis
NAD+ + reduced benzyl viologen
-
-
-
?
1.6.99.3
NADH + methyl viologen
-
701758
Trichomonas vaginalis
NAD+ + reduced methyl viologen
-
-
-
?
1.6.99.3
oxidized ferredoxin + NADH + H+
-
701758
Trichomonas vaginalis
reduced ferredoxin + NAD+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.6.99.3
heterodimer
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6.99.3
650
-
NADH
electron acceptor methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
725
-
NADH
electron acceptor benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
1170
-
NADH
electron acceptor ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.99.3
expressed in Escherichia coli, active protein as well as individual subunits are expressed separately
Trichomonas vaginalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6.99.3
0.06
-
ferricyanide
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.1
-
NADH
electron acceptor benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.17
-
benzyl viologen
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.22
-
NADH
electron acceptor methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.31
-
NADH
electron acceptor ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
0.44
-
methyl viologen
after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.6.99.3
24000
-
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
1.6.99.3
51000
-
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
1.6.99.3
69100
-
gel filtration
Trichomonas vaginalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.6.99.3
-
Trichomonas vaginalis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.6.99.3
24
-
substrate Clostridium acetobutylicum ferredoxin, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
392
-
substrate methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
582
-
substrate benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
604
-
substrate ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.6.99.3
2 ferricyanide + NADH
-
701758
Trichomonas vaginalis
2 ferrocyanide + NAD+ + H+
-
-
-
?
1.6.99.3
NADH + benzyl viologen
-
701758
Trichomonas vaginalis
NAD+ + reduced benzyl viologen
-
-
-
?
1.6.99.3
NADH + methyl viologen
-
701758
Trichomonas vaginalis
NAD+ + reduced methyl viologen
-
-
-
?
1.6.99.3
oxidized ferredoxin + NADH + H+
-
701758
Trichomonas vaginalis
reduced ferredoxin + NAD+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.6.99.3
heterodimer
1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
Trichomonas vaginalis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6.99.3
650
-
NADH
electron acceptor methyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
725
-
NADH
electron acceptor benzyl viologen, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis
1.6.99.3
1170
-
NADH
electron acceptor ferricyanide, after induction with 1.5% arabinose in culture medium
Trichomonas vaginalis