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Literature summary extracted from

  • Okrasa, K.; Levy, C.; Wilding, M.; Goodall, M.; Baudendistel, N.; Hauer, B.; Leys, D.; Micklefield, J.
    Structure-guided directed evolution of alkenyl and arylmalonate decarboxylases (2009), Angew. Chem. Int. Ed. Engl., 48, 7691-7694.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.76 G190A the mutant shows 4.6% relative activity compared to the wild type enzyme Bordetella bronchiseptica
4.1.1.76 G190S/P14V/P15G the mutant shows 1.9% relative activity compared to the wild type enzyme Bordetella bronchiseptica
4.1.1.76 M159C the mutant shows 1.4% relative activity compared to the wild type enzyme using phenylmalonate or methyl(phenyl)propanedioic acid as substrate, the mutant shows 1.7% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate Bordetella bronchiseptica
4.1.1.76 M159G the mutant shows 19% relative activity compared to the wild type enzyme Bordetella bronchiseptica
4.1.1.76 M159S the mutant shows 2.6% relative activity compared to the wild type enzyme Bordetella bronchiseptica
4.1.1.76 M159V the mutant shows 51% relative activity compared to the wild type enzyme Bordetella bronchiseptica
4.1.1.76 P14V/P15G the mutant shows 11% relative activity compared to the wild type enzyme using phenylmalonate as substrate, the mutant shows 1.9% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate, the mutant shows 1.5% relative activity compared to the wild type enzyme using hydroxy(2-methylprop-1-en-1-yl)propanedioic acid as substrate Bordetella bronchiseptica

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.76 benzylphosphonate competitive inhibitor of AMDase Bordetella bronchiseptica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.76 0.3
-
 Phenylmalonate mutant enzyme M159V Bordetella bronchiseptica
4.1.1.76 0.43
-
 Phenylmalonate mutant enzyme M159G Bordetella bronchiseptica
4.1.1.76 0.8
-
 2-vinyl-2-methylmalonic acid mutant enzyme G190A Bordetella bronchiseptica
4.1.1.76 3.5
-
 methyl(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 3.5
-
 Phenylmalonate mutant enzyme P14V/P15G Bordetella bronchiseptica
4.1.1.76 4.1
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 5
-
 Phenylmalonate mutant enzyme M159S Bordetella bronchiseptica
4.1.1.76 7.3
-
 hydroxy(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 7.8
-
 2-vinyl-2-methylmalonic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 9.1
-
 hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 10.7
-
 Phenylmalonate wild type enzyme Bordetella bronchiseptica
4.1.1.76 12.1
-
 Phenylmalonate mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 12.8
-
 methyl[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 14.3
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme G190S/P14V/P15G Bordetella bronchiseptica
4.1.1.76 14.4
-
 (1E)-but-1-en-1-yl(methyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 15.3
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme P14V/P15G Bordetella bronchiseptica
4.1.1.76 26.9
-
 methyl(phenyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 30.2
-
 2-hydroxy-2-phenylmalonic acid wild type enzyme Bordetella bronchiseptica

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.76 Bordetella bronchiseptica Q05115
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.76 (1E)-but-1-en-1-yl(methyl)propanedioic acid
-
 Bordetella bronchiseptica (2S,3Z)-2-methylhex-3-enoic acid + CO2
-
 ?
4.1.1.76 2-amino-2-vinylmalonate
-
 Bordetella bronchiseptica (R)-vinylglycine + CO2
-
 ?
4.1.1.76 2-hydroxy-2-phenylmalonic acid
-
 Bordetella bronchiseptica (2S)-hydroxy(phenyl)ethanoic acid + CO2
-
 ?
4.1.1.76 2-hydroxy-2-phenylmalonic acid
-
 Bordetella bronchiseptica ? + CO2
-
 ?
4.1.1.76 2-vinyl-2-methylmalonic acid
-
 Bordetella bronchiseptica (2S)-2-methylbut-3-enoic acid + CO2
-
 ?
4.1.1.76 2-vinyl-2-methylmalonic acid
-
 Bordetella bronchiseptica ? + CO2
-
 ?
4.1.1.76 amino(phenyl)propanedioic acid
-
 Bordetella bronchiseptica (2S)-amino(phenyl)ethanoic acid + CO2
-
 ?
4.1.1.76 hydroxy(2-methylprop-1-en-1-yl)propanedioic acid
-
 Bordetella bronchiseptica (2S)-2-hydroxy-4-methylpent-3-enoic acid + CO2
-
 ?
4.1.1.76 hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid
-
 Bordetella bronchiseptica (2S)-2-hydroxybut-3-enoic acid + CO2
-
 ?
4.1.1.76 methyl(2-methylprop-1-en-1-yl)propanedioic acid
-
 Bordetella bronchiseptica (2S)-2,4-dimethylpent-3-enoic acid + CO2
-
 ?
4.1.1.76 methyl(phenyl)propanedioic acid
-
 Bordetella bronchiseptica (2S)-2-phenylpropanoic acid + CO2
-
 ?
4.1.1.76 methyl[(1E)-prop-1-en-1-yl]propanedioic acid
-
 Bordetella bronchiseptica (2S,3Z)-2-methylpent-3-enoic acid + CO2
-
 ?
4.1.1.76 Phenylmalonate best substrate Bordetella bronchiseptica Phenylacetate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.76 AMDase
-
 Bordetella bronchiseptica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.76 5.2
-
 methyl(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 20.7
-
 hydroxy(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 20.8
-
 2-vinyl-2-methylmalonic acid mutant enzyme G190A Bordetella bronchiseptica
4.1.1.76 23.4
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 23.9
-
 (1E)-but-1-en-1-yl(methyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 42.8
-
 2-vinyl-2-methylmalonic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 46.2
-
 methyl[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 47.6
-
 hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 94.4
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme G190S/P14V/P15G Bordetella bronchiseptica
4.1.1.76 99.8
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme P14V/P15G Bordetella bronchiseptica
4.1.1.76 101
-
 2-hydroxy-2-phenylmalonic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 246
-
 Phenylmalonate mutant enzyme M159G Bordetella bronchiseptica
4.1.1.76 279
-
 methyl(phenyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 316
-
 Phenylmalonate wild type enzyme Bordetella bronchiseptica
4.1.1.76 386
-
 Phenylmalonate mutant enzyme M159S Bordetella bronchiseptica
4.1.1.76 450
-
 Phenylmalonate mutant enzyme M159V Bordetella bronchiseptica
4.1.1.76 489
-
 Phenylmalonate mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 1143
-
 Phenylmalonate mutant enzyme P14V/P15G Bordetella bronchiseptica

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.76 additional information AMDase does not require cofactors Bordetella bronchiseptica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.76 5.2
-
 benzylphosphonate
-
 Bordetella bronchiseptica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.76 1.5
-
 methyl(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 1.7
-
 (1E)-but-1-en-1-yl(methyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 2.8
-
 hydroxy(2-methylprop-1-en-1-yl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 3.3
-
 2-hydroxy-2-phenylmalonic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 3.6
-
 methyl[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 5.2
-
 hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 5.5
-
 2-vinyl-2-methylmalonic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 5.8
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 6.5
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme P14V/P15G Bordetella bronchiseptica
4.1.1.76 6.6
-
 2-hydroxy-2-phenylmalonic acid mutant enzyme G190S/P14V/P15G Bordetella bronchiseptica
4.1.1.76 10.4
-
 methyl(phenyl)propanedioic acid wild type enzyme Bordetella bronchiseptica
4.1.1.76 25.2
-
 2-vinyl-2-methylmalonic acid mutant enzyme G190A Bordetella bronchiseptica
4.1.1.76 29.6
-
 Phenylmalonate wild type enzyme Bordetella bronchiseptica
4.1.1.76 40.4
-
 Phenylmalonate mutant enzyme M159C Bordetella bronchiseptica
4.1.1.76 77.2
-
 Phenylmalonate mutant enzyme M159S Bordetella bronchiseptica
4.1.1.76 326
-
 Phenylmalonate mutant enzyme P14V/P15G Bordetella bronchiseptica
4.1.1.76 570
-
 Phenylmalonate mutant enzyme M159G Bordetella bronchiseptica
4.1.1.76 1499
-
 Phenylmalonate mutant enzyme M159V Bordetella bronchiseptica