EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.2.6 | analysis | a novel method to quantify L/F transferase activity by matrix assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF, is reported | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.2.6 | into the vector pCA24N | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.6 | 2.2 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-azido phenylalanine, Km apparent | Escherichia coli | |
2.3.2.6 | 3.2 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, phenylalanine, Km apparent | Escherichia coli | |
2.3.2.6 | 4.3 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Km apparent | Escherichia coli | |
2.3.2.6 | 18 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-acetyl-N-(tert-butoxycarbonyl)-L-phenylalanine, Km apparent | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.6 | L-leucyl-tRNALeu + protein | Escherichia coli | - |
tRNALeu + L-leucyl-protein | - |
? | |
2.3.2.6 | L-phenylalanyl-tRNA + protein | Escherichia coli | - |
tRNA + L-phenylalanyl-protein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.6 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.6 | L-leucyl-tRNA + REPGLCTWQSLR | substrate peptide | Escherichia coli | tRNA + LREPGLCTWQSLR | - |
? | |
2.3.2.6 | L-leucyl-tRNALeu + protein | - |
Escherichia coli | tRNALeu + L-leucyl-protein | - |
? | |
2.3.2.6 | L-phenylalanyl-tRNA + protein | - |
Escherichia coli | tRNA + L-phenylalanyl-protein | - |
? | |
2.3.2.6 | L-phenylalanyl-tRNA + REPGLCTWQSLR | substrate peptide | Escherichia coli | tRNA + FREPGLCTWQSLR | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.6 | L/F transferase | - |
Escherichia coli |
2.3.2.6 | leucyl/phenylalanyl-tRNA-protein transferase | - |
Escherichia coli |
2.3.2.6 | leucyltransferase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.6 | 37 | - |
peptide bond formation assay | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.6 | 0.00183 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-azido phenylalanine, Kcat apparent | Escherichia coli | |
2.3.2.6 | 0.00283 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-acetyl-N-(tert-butoxycarbonyl)-L-phenylalanine, Kcat apparent | Escherichia coli | |
2.3.2.6 | 0.00333 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, 4-bromo phenylalanine, Kcat apparent | Escherichia coli | |
2.3.2.6 | 0.00367 | - |
REPGLCTWQSLR | amino acid residue on the aminoacyl-tRNA(Phe) transferred to the peptide substrate, phenylalanine, Kcat apparent | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.2.6 | 7.5 | - |
peptide bond formation assay | Escherichia coli |