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Literature summary extracted from

  • Rao, F.; Pasunooti, S.; Ng, Y.; Zhuo, W.; Lim, L.; Liu, A.W.; Liang, Z.X.
    Enzymatic synthesis of c-di-GMP using a thermophilic diguanylate cyclase (2009), Anal. Biochem., 389, 138-142.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.65 synthesis the thermophilic enzyme is a valuable tool for c-di-GMP synthesis as well as the preparation of c-di-GMP derivatives Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.65 a DNA construct encoding the C-terminus of the TM1788 gene is cloned into the vector pET28b+ for expression in Escherichia col BL21DE3 cells Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.65 R158A mutation of a key residue in the putative regulatory I-site, product inhibition is substantially alleviated Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.65 Mg2+
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.65 21000
-
 determined by SDS-PAGE Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.65 GTP Thermotoga maritima
-
 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.65 Thermotoga maritima Q9X2A8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.65 using Ni2+-nitrilotriacetic acid resin and a gel filtration coumn Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.65 GTP
-
 Thermotoga maritima diphosphate + cyclic di-3',5'-guanylate
-
 ?
2.7.7.65 GTP + GTP
-
 Thermotoga maritima 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.65 dimer wild-type Thermotoga maritima
2.7.7.65 monomer R158A mutant Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.7.65 DGC
-
 Thermotoga maritima
2.7.7.65 diguanylate cyclase
-
 Thermotoga maritima
2.7.7.65 tDGC
-
 Thermotoga maritima
2.7.7.65 thermophilic diguanylate cyclase domain protein
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.65 55
-
 R158A mutant Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.65 0.0433
-
 GTP R158A mutant Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.65 7.5
-
 R158A mutant Thermotoga maritima