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Literature summary extracted from

  • Van Pelt, S.; Van Rantwijk, F.; Sheldon, R.
    Synthesis of aliphatic (S)-alpha-hydroxycarboxylic amides using a one-pot bienzymatic cascade of immobilised oxynitrilase and nitrile hydratase (2009), Adv. Synth. Catal., 351, 397-404.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.84 nitrile hydratase cross-linked enzyme aggregates are sensitive to water-immiscible organic solvents as well as to aldehydes and hydrogen cyanide, but are remarkably stable and show useful activity in acidic aqueous environments of pH 4-5 Nitriliruptor alkaliphilus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta
-
-
-
4.2.1.84 Nitriliruptor alkaliphilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 cyanide + prop-2-enal
-
 Manihot esculenta (2S)-2-hydroxy-3-methylbutanenitrile
-
 ?
4.1.2.47 cyanide + propanal
-
 Manihot esculenta (2S)-2-hydroxybutanenitrile
-
 ?
4.2.1.84 (2R)-2-hydroxy-3-methylbutanenitrile + H2O
-
 Nitriliruptor alkaliphilus (2R)-2-hydroxy-3-methylbutanamide
-
 ?
4.2.1.84 (2R)-2-hydroxybut-3-enenitrile + H2O
-
 Nitriliruptor alkaliphilus (2R)-2-hydroxybut-3-enamide
-
 ?
4.2.1.84 (2R)-2-hydroxybutanenitrile + H2O
-
 Nitriliruptor alkaliphilus (2R)-2-hydroxybutanamide
-
 ?
4.2.1.84 (2R)-2-hydroxyhexanenitrile + H2O
-
 Nitriliruptor alkaliphilus (2R)-2-hydroxyhexanamide
-
 ?
4.2.1.84 (2R)-2-hydroxypentanenitrile + H2O
-
 Nitriliruptor alkaliphilus (2R)-2-hydroxypentanamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-HNL
-
 Manihot esculenta
4.1.2.47 (S)-selective HNL
-
 Manihot esculenta
4.2.1.84 NHase
-
 Nitriliruptor alkaliphilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 8
-
 around pH 8.0 Nitriliruptor alkaliphilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.84 4 12 nitrile hydratase cross-linked enzyme aggregates are stable at pH values 2-4 units below the optimum (pH 8.0) and still have a useful activity under these conditions, retains about 20% activity at pH 4, 40% activity at pH 5.0, 65% activity at pH 6.0, 90% activity at pH 7.0, 9.0 and 10.0, 85% activity at pH 12.0 Nitriliruptor alkaliphilus