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Literature summary extracted from

  • Pietkiewicz, J.; Bednarz-Misa, I.; Jermakow, K.; Gamian, A.
    Enolase from Klebsiella pneumoniae and human muscle cells II. Kinetic parameters and sensitivity to fluoride and phosphate inhibitors (2009), Adv. Clin. Exp. Med., 18, 221-233.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 fluoride the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level Homo sapiens
4.2.1.11 fluoride the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level Klebsiella pneumoniae
4.2.1.11 Mg2+ inhibitory in excess Homo sapiens
4.2.1.11 Mg2+ inhibitory in excess Klebsiella pneumoniae
4.2.1.11 Mn2+ inhibitory in excess Homo sapiens
4.2.1.11 Mn2+ inhibitory in excess Klebsiella pneumoniae
4.2.1.11 phosphate at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition Homo sapiens
4.2.1.11 phosphate at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition Klebsiella pneumoniae
4.2.1.11 Zn2+ inhibitory in excess Homo sapiens
4.2.1.11 Zn2+ inhibitory in excess Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.199
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 0.425
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 0.657
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 0.702
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytoplasm
-
 Homo sapiens 5737
-
4.2.1.11 cytoplasm
-
 Klebsiella pneumoniae 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found Homo sapiens
4.2.1.11 Mg2+ maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Homo sapiens
-
-
-
4.2.1.11 Klebsiella pneumoniae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Homo sapiens phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Klebsiella pneumoniae phosphoenolpyruvate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 2-phospho-D-glycerate hydrolyase
-
 Homo sapiens
4.2.1.11 2-phospho-D-glycerate hydrolyase
-
 Klebsiella pneumoniae
4.2.1.11 enolase
-
 Homo sapiens
4.2.1.11 enolase
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.11 15.47
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 34.07
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 52.7
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 81.68
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 6.8
-
-
 Homo sapiens
4.2.1.11 7.8
-
-
 Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.11 0.32
-
 phosphate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 0.9
-
 phosphate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 1.266
-
 Mn2+ in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 1.58
-
 Zn2+ in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 2.2
-
 fluoride at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 2.34
-
 Zn2+ in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 3.04
-
 Mn2+ in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 3.249
-
 Mg2+ in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 14.37
-
 Mg2+ in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 18.8
-
 fluoride at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.11 23.5
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 48.5
-
 phosphoenolpyruvate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens
4.2.1.11 124
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl Klebsiella pneumoniae
4.2.1.11 410
-
 2-phospho-D-glycerate in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl Homo sapiens