BRENDA - Enzyme Database

Enolase from Klebsiella pneumoniae and human muscle cells II. Kinetic parameters and sensitivity to fluoride and phosphate inhibitors

Pietkiewicz, J.; Bednarz-Misa, I.; Jermakow, K.; Gamian, A.; Adv. Clin. Exp. Med. 18, 221-233 (2009)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
fluoride
the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level
Homo sapiens
4.2.1.11
fluoride
the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level
Klebsiella pneumoniae
4.2.1.11
Mg2+
inhibitory in excess
Homo sapiens
4.2.1.11
Mg2+
inhibitory in excess
Klebsiella pneumoniae
4.2.1.11
Mn2+
inhibitory in excess
Homo sapiens
4.2.1.11
Mn2+
inhibitory in excess
Klebsiella pneumoniae
4.2.1.11
phosphate
at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition
Homo sapiens
4.2.1.11
phosphate
at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition
Klebsiella pneumoniae
4.2.1.11
Zn2+
inhibitory in excess
Homo sapiens
4.2.1.11
Zn2+
inhibitory in excess
Klebsiella pneumoniae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.199
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
0.425
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
0.657
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
0.702
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Klebsiella pneumoniae
5737
-
4.2.1.11
cytoplasm
-
Homo sapiens
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found
Homo sapiens
4.2.1.11
Mg2+
maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found
Klebsiella pneumoniae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Homo sapiens
-
-
-
4.2.1.11
Klebsiella pneumoniae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701578
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
701578
Klebsiella pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
15.47
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
34.07
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
52.7
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
81.68
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Homo sapiens
4.2.1.11
7.8
-
-
Klebsiella pneumoniae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.32
-
phosphate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
0.9
-
phosphate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
1.266
-
Mn2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
1.58
-
Zn2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
2.2
-
fluoride
at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
2.34
-
Zn2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
3.04
-
Mn2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
3.249
-
Mg2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
14.37
-
Mg2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
18.8
-
fluoride
at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
fluoride
the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level
Homo sapiens
4.2.1.11
fluoride
the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level
Klebsiella pneumoniae
4.2.1.11
Mg2+
inhibitory in excess
Homo sapiens
4.2.1.11
Mg2+
inhibitory in excess
Klebsiella pneumoniae
4.2.1.11
Mn2+
inhibitory in excess
Homo sapiens
4.2.1.11
Mn2+
inhibitory in excess
Klebsiella pneumoniae
4.2.1.11
phosphate
at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition
Homo sapiens
4.2.1.11
phosphate
at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition
Klebsiella pneumoniae
4.2.1.11
Zn2+
inhibitory in excess
Homo sapiens
4.2.1.11
Zn2+
inhibitory in excess
Klebsiella pneumoniae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
0.32
-
phosphate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
0.9
-
phosphate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
1.266
-
Mn2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
1.58
-
Zn2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
2.2
-
fluoride
at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
2.34
-
Zn2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
3.04
-
Mn2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
3.249
-
Mg2+
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
14.37
-
Mg2+
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
18.8
-
fluoride
at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.199
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
0.425
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
0.657
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
0.702
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Klebsiella pneumoniae
5737
-
4.2.1.11
cytoplasm
-
Homo sapiens
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found
Homo sapiens
4.2.1.11
Mg2+
maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found
Klebsiella pneumoniae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701578
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
701578
Klebsiella pneumoniae
phosphoenolpyruvate + H2O
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
15.47
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
34.07
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
52.7
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
81.68
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Homo sapiens
4.2.1.11
7.8
-
-
Klebsiella pneumoniae
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
23.5
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
48.5
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
124
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
410
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
23.5
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
48.5
-
phosphoenolpyruvate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens
4.2.1.11
124
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl
Klebsiella pneumoniae
4.2.1.11
410
-
2-phospho-D-glycerate
in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl
Homo sapiens