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Literature summary extracted from
- Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase (2010), Acta Crystallogr. Sect. F, 66, 320-323.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expressed in Escherichia coli Origami B (DE3) cells | Oryza sativa |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | thioredoxin/His6-tagged 4BGlu12 fusion protein complexed with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside, hanging drop vapor diffusion method, using 19% polyethylene glycol (3350 or 2000) in 0.1 M Tris-HCl pH 8.5, 0.16 M NaCl, at 15°C | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Oryza sativa | Q75I93 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | Sepharose 6 immobilized metal-ion affinity chromatography on cobalt | Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside + H2O | - |
Oryza sativa | ? | - |
? |  |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Oryza sativa | 4-nitrophenol + beta-D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | 4BGlu12 | - |
Oryza sativa |
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