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Literature summary extracted from

  • Sakamoto, Y.; Ike, M.; Tanaka, N.; Suzuki, Y.; Ogasawara, W.; Okada, H.; Nonaka, T.; Morikawa, Y.; Nakamura, K.T.
    Crystallization and preliminary X-ray crystallographic studies of an exo-beta-D-glucosaminidase from Trichoderma reesei (2010), Acta Crystallogr. Sect. F, 66, 309-312.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expressed in Pichia pastoris Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.165 hanging drop vapor diffusion method, using 14% (w/v) PEG 8000 and 0.2 M ammonium acetate in 0.1 M cacodylate buffer pH 7.0 for enzyme form Gls93-F1 or 20% (w/v) PEG 4000 and 0.2 M sodium isothiocyanate for enzyme form Gls93-F2 Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.165 extracellular
-
 Trichoderma reesei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.165 100000
-
 form Gls93-F2, SDS-PAGE Trichoderma reesei
3.2.1.165 105000
-
 form Gls93-F1, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.165 chitosan + H2O Trichoderma reesei
-
 D-glucosamine + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Trichoderma reesei Q4R1C4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 ammonium sulfate precipitation, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Sephacryl S-100 gel filtration Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitosan + H2O
-
 Trichoderma reesei D-glucosamine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Trichoderma reesei
3.2.1.165 GlcNase
-
 Trichoderma reesei
3.2.1.165 Gls93
-
 Trichoderma reesei