Literature summary extracted from
Sakurai, K.; Shimada, H.; Hayashi, T.; Tsukihara, T.
Substrate binding induces structural changes in cytochrome P450cam (2009), Acta Crystallogr. Sect. F, 65, 80-83.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.1 |
expressed in Escherichia coli strain JM109 |
Pseudomonas putida |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.14.15.1 |
two ferric P450cam structures partially complexed with (+)-camphor, by sitting-drop vapour-diffusion method, at 1.3 A (soaked crystals) or 1.35 A (unsoaked crystals) resolution. Belongs to space group P43212, unsoaked crystals have unit-cell parameters of a = b = 63.38, c = 247.30, whereas soaked crystals have unit-cell parameters of a = b = 63.61 and c = 250.39. Structure of the unsoaked P450cam shows an active site that is partially occupied by (+)-camphor and a water molecule liganded to the haem iron and rotamers of Thr101. (+)-Camphor-bound form is the major component and the water-bound form is the minor component. In the soaked P450cam, the population of the major component increases, while the minor component decreases. (+)-Camphor binding induces rotation of Thr101 to form a hydrogen bond that acts as a hydrogen donor to a peripheral haem propionate. This bonding contributes to redox-potential change |
Pseudomonas putida |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
Fe2+ |
- |
Pseudomonas putida |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.1 |
Pseudomonas putida |
P00183 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.1 |
to homogeneity by SDSPAGE |
Pseudomonas putida |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.15.1 |
-80°C, 50 mM potassium phosphate buffer, pH 7.4, 50 mM KCl, 1 mM (+)-camphor |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.1 |
(+)-camphor + O2 + reduced putidaredoxin |
- |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.1 |
cytochrome p450cam |
- |
Pseudomonas putida |
1.14.15.1 |
P450cam |
- |
Pseudomonas putida |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
putidaredoxin |
- |
Pseudomonas putida |
|