BRENDA - Enzyme Database

Crystallization and preliminary X-ray analysis of human liver alpha-enolase

Wang, J.; Zhou, Y.F.; Li, L.F.; Su, X.D.; Acta Crystallogr. Sect. F 65, 288-290 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop vapor diffusion method, using 0.1 M ammonium acetate, 0.1 M bis-tris pH 5.5, 20% (w/v) polyethylene glycol monomethyl ether 2000
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
calculated from amino acid sequence
Homo sapiens
4.2.1.11
50000
-
gel filtration
Homo sapiens
4.2.1.11
51000
-
His-tagged enzyme, SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Homo sapiens
P06733
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
HiTrap Ni2+-chelating column chromatography and Superdex 75 gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701517
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
monomer
1 * 50000, gel filtration
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop vapor diffusion method, using 0.1 M ammonium acetate, 0.1 M bis-tris pH 5.5, 20% (w/v) polyethylene glycol monomethyl ether 2000
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
calculated from amino acid sequence
Homo sapiens
4.2.1.11
50000
-
gel filtration
Homo sapiens
4.2.1.11
51000
-
His-tagged enzyme, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
HiTrap Ni2+-chelating column chromatography and Superdex 75 gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
701517
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
monomer
1 * 50000, gel filtration
Homo sapiens