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Literature summary extracted from

  • Wang, J.; Zhou, Y.F.; Li, L.F.; Su, X.D.
    Crystallization and preliminary X-ray analysis of human liver alpha-enolase (2009), Acta Crystallogr. Sect. F, 65, 288-290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.11 hanging drop vapor diffusion method, using 0.1 M ammonium acetate, 0.1 M bis-tris pH 5.5, 20% (w/v) polyethylene glycol monomethyl ether 2000 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 47000
-
 calculated from amino acid sequence Homo sapiens
4.2.1.11 50000
-
 gel filtration Homo sapiens
4.2.1.11 51000
-
 His-tagged enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Homo sapiens P06733
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 HiTrap Ni2+-chelating column chromatography and Superdex 75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Homo sapiens phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 monomer 1 * 50000, gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 2-phospho-D-glycerate hydrolyase
-
 Homo sapiens
4.2.1.11 alpha-enolase
-
 Homo sapiens
4.2.1.11 ENO1
-
 Homo sapiens
4.2.1.11 enolase
-
 Homo sapiens