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Literature summary extracted from

  • Atkinson, S.C.; Dobson, R.C.; Newman, J.M.; Gorman, M.A.; Dogovski, C.; Parker, M.W.; Perugini, M.A.
    Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate (2009), Acta Crystallogr. Sect. F, 65, 253-255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3) Clostridium botulinum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46% Clostridium botulinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.7 31530
-
 purified enzyme, mass spectrometry Clostridium botulinum
4.3.3.7 31650
-
 mass spectrometry Clostridium botulinum

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Clostridium botulinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 by anion-exchange chromatography, hydrophobic interaction and gel filtration Clostridium botulinum

Storage Stability

EC Number Storage Stability Organism
4.3.3.7 -20°C, 20 mM Tris-HCl buffer, pH 8.0 Clostridium botulinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 L-aspartate 4-semialdehyde + pyruvate
-
 Clostridium botulinum dihydrodipicolinate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.7 tetramer crystallography Clostridium botulinum

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DapA
-
 Clostridium botulinum
4.3.3.7 DHDPS
-
 Clostridium botulinum
4.3.3.7 dihydrodipicolinate synthase
-
 Clostridium botulinum