Literature summary extracted from
Strange, R.W.; Antonyuk, S.V.; Ellis, M.J.; Bessho, Y.; Kuramitsu, S.; Yokoyama, S.; Hasnain, S.S.
The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603) (2009), Acta Crystallogr. Sect. F, 65, 1214-1217.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.3.1.6 |
expression of MJ1603 in Escherichia coli strain Rosetta (DE3) |
Methanocaldococcus jannaschii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.1.6 |
purified recombinant MJ1603, microbatch-under-oil method, 0.0005 ml of 8.1 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, containing 200 mM NaCl are mixed with 0.0005 ml of crystallization reagent, consisting of 0.1 M acetate, pH 4.5 containing 40% v/v 1,2-propanediol and 0.05 M calcium acetate, 18°C. The mixture is covered with 0.015 ml silicone and paraffin oil, X-ray diffraction structure determination and analysis at 1.78 A resolution, molecular replacement and modeling |
Methanocaldococcus jannaschii |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.3.1.6 |
D-ribose 5-phosphate |
Methanocaldococcus jannaschii |
- |
D-ribulose 5-phosphate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.1.6 |
Methanocaldococcus jannaschii |
Q58998 |
strain DSM 2661 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.3.1.6 |
recombinant MJ1603 from Escherichia coli strain Rosetta (DE3) by anion exchange and hydroxyapatite chromatography, and gel filtration |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.3.1.6 |
D-ribose 5-phosphate |
- |
Methanocaldococcus jannaschii |
D-ribulose 5-phosphate |
- |
r |
|
5.3.1.6 |
D-ribose 5-phosphate |
the open configuration of ribose 5-phosphate is required for isomerization and is reversibly converted to ribulose 5-phosphate |
Methanocaldococcus jannaschii |
D-ribulose 5-phosphate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.3.1.6 |
tetramer |
RpiA crystal structure, overview |
Methanocaldococcus jannaschii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.3.1.6 |
MJ1603 |
- |
Methanocaldococcus jannaschii |
5.3.1.6 |
RpiA |
- |
Methanocaldococcus jannaschii |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.3.1.6 |
metabolism |
two evolutionary distinct forms of the enzyme, RpiA and RpiB, with different amino acid sequences and molecular weights exist, that both catalyze the reversible conversion of ribose 5-phosphate to ribulose 5-phosphate. RpiA is found in the bacterial, plant and animal kingdoms, whereas RpiB is less widespread and is found in bacterial sources |
Methanocaldococcus jannaschii |