EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.5 | 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase | - |
Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.54 | expression of enzyme with affinity purification tagwith plasmid pKTDS-HN in Escherichia coli KA13 | Mycobacterium tuberculosis |
5.4.99.5 | expressed in Escherichia coli strain KA13 | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.54 | in complex with chorismate mutase, hanging-drop method, in 20 mM BTP, pH 7.5, 150 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine hydrochloride, 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, crystallization after 2 months with no ammonium sulfate and 0.1 M Tris-HCl, pH 7.9-8.0 and PEG 400 or glycerol | Mycobacterium tuberculosis |
5.4.99.5 | alone and in complex with 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, hanging drop vapor diffusion method, using 25% (w/v) PEG 1500 and 0.1 M MMT buffer (L-malic acid, MES, Tris) pH 8.0-9.0 | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 51830 | - |
expected mass, calculated from the 472 amino acids | Mycobacterium tuberculosis |
2.5.1.54 | 51830 | - |
observed by ESI-MS | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Mycobacterium tuberculosis | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.54 | Mycobacterium tuberculosis | O53512 | - |
- |
5.4.99.5 | Mycobacterium tuberculosis | P9WIC1 | - |
- |
5.4.99.5 | Mycobacterium tuberculosis H37Rv | P9WIC1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.54 | cells centrifuged and resuspended in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP (tris(2-carboxyethyl)phosphine hydrochloride), 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, cell lysis with lysozyme and sonication, centrifugation, crude extract subjected to Ni-NTA agarose chromatography, elution with 250 mM imidazole in buffer, further purification by preparative FPLC HiLoad 26/60 Superdex 75 gel-filtration column | Mycobacterium tuberculosis |
5.4.99.5 | ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and Superdex 75 gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Mycobacterium tuberculosis | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
5.4.99.5 | Chorismate | the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate | Mycobacterium tuberculosis | Prephenate | - |
? | |
5.4.99.5 | Chorismate | the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate | Mycobacterium tuberculosis H37Rv | Prephenate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.54 | 3-deoxy-7-phosphoheptulonate synthase | - |
Mycobacterium tuberculosis |
2.5.1.54 | DAHP synthase | - |
Mycobacterium tuberculosis |
2.5.1.54 | MtDS | - |
Mycobacterium tuberculosis |
5.4.99.5 | Rv0948c | - |
Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.54 | metabolism | initial step in the shikimate biosynthesis pathway, enhances the catalytic efficiency of chorismate mutase in a noncovalent enzyme complex | Mycobacterium tuberculosis |