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Literature summary extracted from

  • Goncalves, S.; Borges, N.; Santos, H.; Matias, P.M.
    Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27 (2009), Acta Crystallogr. Sect. F, 65, 1014-1017.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.217 expressed in Escherichia coli BL21 Rosetta cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.217 hanging drop vapor diffusion method, using 0.2 M magnesium acetate, 0.1 M sodium cacodylate pH 6.5, 30-35% MPD with 600 mM ZnCl2, the addition of Zn2+ to the crystallization buffer is essential in order to obtain crystals Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.217 43600
-
 x * 43600, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus
-
 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.217 Thermus thermophilus
-
-
-
2.4.1.217 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.217 Q-Sepharose column chromatography, Resource Q column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?
2.4.1.217 GDP-mannose + 3-phospho-D-glycerate
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.217 ? x * 43600, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.217 mannosyl-3-phosphoglycerate synthase
-
 Thermus thermophilus
2.4.1.217 MPGS
-
 Thermus thermophilus