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Literature summary extracted from

  • Cicmil, N.
    Crystallization and preliminary X-ray crystallographic characterization of TrmFO, a folate-dependent tRNA methyltransferase from Thermotoga maritima (2008), Acta Crystallogr. Sect. F, 64, 193-195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.74 overexpression in Escherichia coli strain BL21(DE3) Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.74 purified recombinant enzyme, from 25% v/v PEG 4000, 100 mM NaCl and sodium citrate buffer, pH 5.0, at 291 K using the hanging-drop vapor-diffusion method in 0.0016 ml with 8 mg/ml protein, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 Thermotoga maritima TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Thermotoga maritima
-
 gene trmFO
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.74 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion and cation exchange chromatography and gel filtration to over 95% purity Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 TrmFO is a methyltransferase that catalyzes the formation of 5-methyluridine or ribothymidine at position 54 in tRNA in some Gram-positive bacteria Thermotoga maritima tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
-
 ?
2.1.1.74 N5,N10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2
-
 Thermotoga maritima tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.74 TRMFO
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.74 FAD dependent on Thermotoga maritima