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Literature summary extracted from

  • Atkin, K.E.; Reiss, R.; Turner, N.J.; Brzozowski, A.M.; Grogan, G.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of monoamine oxidase from Aspergillus niger (2008), Acta Crystallogr. Sect. F, 64, 182-185.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 overexpression of His-tagged wild-type and mutants enzymes in Escherichia coli strain Rosetta2(DE3) Aspergillus niger

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.21 purified recombinant genetic variants MAO-N-3 and MAO-N-5, from 10% w/v PEG 3350, 0.2 M proline, 0.1 M HEPES, pH 7.5, or 10% w/v PEG 5000 MME, 5% v/v tacsimate, 0.1 M HEPES, pH 7.0, with no difference in diffraction quality between the crystals from the two conditions, X-ray diffraction structure determination and analysis at 2.45 A and 1.85 A resolution, respectively Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.21 additional information genetic variants of MAO-N produced by directed evolution possess altered substrate specificity, e.g. MAO-N-3 and MAO-N-5 Aspergillus niger
1.4.3.21 N336S/M348K/I246M genetic variant MOA-N-3 exhibits improved activity towards a range of amine substrates compared to the wild-type enzyme, including chiral secondary amines Aspergillus niger
1.4.3.21 N336S/M348K/I246M/T384N/D385S genetic variant MOA-N-5 shows improved activity and enantioselectivity towards a broad range of tertiary amines compared to the wild-type enzyme Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 rasagiline ethanedisulfonate inhibits MAO-B Aspergillus niger
1.4.3.21 tacsimate
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 recombinant His-tagged wild-type and mutants enzymes from Escherichia coli strain Rosetta2(DE3), removal of the His tag Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 additional information MAO-N is an FAD-dependent enzyme that catalyses the conversion of terminal amines to their corresponding aldehyde Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.21 tetramer
-
 Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 MAO-N
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 FAD dependent on Aspergillus niger