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Literature summary extracted from

  • Chetnani, B.; Das, S.; Kumar, P.; Surolia, A.; Vijayan, M.
    Mycobacterium tuberculosis pantothenate kinase: possible changes in location of ligands during enzyme action (2009), Acta Crystallogr. Sect. D, 65, 312-325.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33
-
 Mycobacterium tuberculosis
2.7.1.33 His-tag, expressed in Escherichia coli BL21 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.33 0.115
-
 ATP Tris-HCl buffer, pH 8.0 Escherichia coli
2.7.1.33 0.151
-
 ATP Tris-HCl buffer, pH8.0 Mycobacterium tuberculosis
2.7.1.33 0.406
-
 ATP citrate buffer, pH 6.0 Escherichia coli
2.7.1.33 0.616
-
 ATP citrate buffer, pH6.0 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Escherichia coli
-
-
-
2.7.1.33 Mycobacterium tuberculosis P9WPA7
-
-
2.7.1.33 Mycobacterium tuberculosis H37Rv P9WPA7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33
-
 Escherichia coli
2.7.1.33
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 pantothenate + ATP
-
 Escherichia coli phosphopantothenate + ADP
-
 ?
2.7.1.33 pantothenate + ATP
-
 Mycobacterium tuberculosis phosphopantothenate + ADP
-
 ?
2.7.1.33 pantothenate + ATP
-
 Mycobacterium tuberculosis H37Rv phosphopantothenate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 pantothenate kinase
-
 Escherichia coli
2.7.1.33 pantothenate kinase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.33 0.052
-
 ATP citrate buffer, pH6.0 Mycobacterium tuberculosis
2.7.1.33 0.071
-
 ATP citrate buffer, pH 6.0 Escherichia coli
2.7.1.33 0.411
-
 ATP Tris-HCl buffer, pH8.0 Mycobacterium tuberculosis
2.7.1.33 1.14
-
 ATP Tris-HCl buffer, pH 8.0 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.33 0.08
-
 ATP citrate buffer, pH6.0 Mycobacterium tuberculosis
2.7.1.33 0.17
-
 ATP citrate buffer, pH 6.0 Escherichia coli
2.7.1.33 2.72
-
 ATP Tris-HCl buffer, pH8.0 Mycobacterium tuberculosis
2.7.1.33 9.91
-
 ATP Tris-HCl buffer, pH 8.0 Escherichia coli