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Literature summary extracted from

  • Gay, S.C.; Segel, I.H.; Fisher, A.J.
    Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity (2009), Acta Crystallogr. Sect. D, 65, 1021-1031.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed in Escherichia coli BL21(DE3) Thiobacillus denitrificans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 hanging-drop vapor diffusion method Thiobacillus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 60500
-
 6 * 60500, calculated, crystall structure analysis Thiobacillus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Thiobacillus denitrificans Q3SM86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 immobilized metal ion affinity chromatography (Ni2+) Thiobacillus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 MgATP2- + adenosine 5'-phosphosulfate
-
 Thiobacillus denitrificans MgADP- + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 homohexamer 6 * 60500, calculated, crystall structure analysis Thiobacillus denitrificans

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 MgATP:APS 3'-phosphotransferase
-
 Thiobacillus denitrificans