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Literature summary extracted from

  • Shah, A.A.; Hasan, F.; Hameed, A.; Ahmed, S.
    A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase (2008), Acta Biol. Hung., 59, 489-499.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.75 biotechnology the optimum production of PHB depolymerase is observed at pH 8 and 7, at 45°C, 1% substrate concentration and in the presence of lactose as an additional carbon source Streptomyces kashimirensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 37000
-
 SDS-PAGE shows two bands Streptomyces kashimirensis
3.1.1.75 45000
-
 SDS-PAGE shows two bands Streptomyces kashimirensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Streptomyces kashimirensis
-
 AF1
-
3.1.1.75 Streptomyces kashimirensis AF1
-
 AF1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75 Streptomyces kashimirensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly (3-hydroxybutyrate) + H2O
-
 Streptomyces kashimirensis ?
-
 ?
3.1.1.75 poly (3-hydroxybutyrate) + H2O
-
 Streptomyces kashimirensis AF1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.75 PHB-depolymerase
-
 Streptomyces kashimirensis
3.1.1.75 poly(3-hydroxybutyrate) depolymerase
-
 Streptomyces kashimirensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 30
-
 assay at Streptomyces kashimirensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8
-
 assay at Streptomyces kashimirensis