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Literature summary extracted from
- Molecular cloning, genetic organization of gene cluster encoding phenol hydroxylase and catechol 2,3-dioxygenase in Alcaligenes faecalis IS-46 (2008), World J. Microbiol. Biotechnol., 24, 1687-1695.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.2 | expression in Escherichia coli LE392 | Alcaligenes faecalis |
| 1.14.13.7 | a 7.6 kb SalI fragment of pZC1115 containing LmPH gene subcloned to pUC19 vector, resulting in recombinant plasmid pME08. Expressed in Escherichia coli DH5alpha | Alcaligenes faecalis |
| 1.14.13.7 | a 7.6 kb SalI fragment of pZC1115 containing LmPH gene subcloned to pUC19 vector, resulting in recombinant plasmid pME08. Expressed in Escherichia coli DH5alpha | Alcaligenes sp. IS-46 |
| 1.14.13.7 | expression in Escherichia coli LE392 | Alcaligenes faecalis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.7 | additional information | strain IS-67 could not grow in medium supplemented with 1000 mg/l of phenol | Alcaligenes faecalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.2 | Alcaligenes faecalis | A5HMH3 | - |
- |
| 1.13.11.2 | Alcaligenes faecalis IS-46 | A5HMH3 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | - |
- |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMH6 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMH7 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMH8 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMH9 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMI0 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis | A5HMI1 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMH6 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMH7 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMH8 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMH9 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMI0 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-46 | A5HMI1 | - |
- |
| 1.14.13.7 | Alcaligenes faecalis IS-67 | - |
- |
- |
| 1.14.13.7 | Alcaligenes sp. IS-46 | Q6V9W5 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.7 | additional information | isolated from Alcaligenes faecalis strains from activated sludge of an industrial coking wastewater treatment plant | Alcaligenes faecalis | - |
| 1.14.13.7 | additional information | isolated from Alcaligenes faecalis strains from activated sludge of an industrial coking wastewater treatment plant | Alcaligenes sp. IS-46 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.2 | phenol + O2 | utilize phenol as sole carbon and energy source, concentration of phenol diversify from 25 mg/l to 1000 mg/l, assay at 28°C, pH 6.8-7.0 | Alcaligenes faecalis | 2-hydroxymuconic semialdehyde | - |
? |  |
| 1.13.11.2 | phenol + O2 | utilize phenol as sole carbon and energy source, concentration of phenol diversify from 25 mg/l to 1000 mg/l, assay at 28°C, pH 6.8-7.0 | Alcaligenes faecalis IS-46 | 2-hydroxymuconic semialdehyde | - |
? | |
| 1.14.13.7 | phenol + NAD(P)H + H+ + O2 | - |
Alcaligenes faecalis | catechol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.7 | phenol + NAD(P)H + H+ + O2 | - |
Alcaligenes sp. IS-46 | catechol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.7 | phenol + NAD(P)H + H+ + O2 | - |
Alcaligenes faecalis IS-67 | catechol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.7 | phenol + O2 | assay at 28°C, pH 6.8-7.0, concentration of phenol diversify from 25 mg/l to 800 mg/l | Alcaligenes faecalis | ? | - |
? | |
| 1.14.13.7 | phenol + O2 | utilize phenol as sole carbon and energy source, concentration of phenol diversify from 25 mg/l to 1000 mg/l, assay at 28°C, pH 6.8-7.0 | Alcaligenes faecalis | ? | - |
? | |
| 1.14.13.7 | phenol + O2 | assay at 28°C, pH 6.8-7.0, concentration of phenol diversify from 25 mg/l to 800 mg/l | Alcaligenes faecalis IS-67 | ? | - |
? | |
| 1.14.13.7 | phenol + O2 | utilize phenol as sole carbon and energy source, concentration of phenol diversify from 25 mg/l to 1000 mg/l, assay at 28°C, pH 6.8-7.0 | Alcaligenes faecalis IS-46 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.2 | C23o | - |
Alcaligenes faecalis |
| 1.13.11.2 | catechol 2,3-dioxygenase | - |
Alcaligenes faecalis |
| 1.14.13.7 | LmPH | - |
Alcaligenes faecalis |
| 1.14.13.7 | LmPH | - |
Alcaligenes sp. IS-46 |
| 1.14.13.7 | Mph | - |
Alcaligenes faecalis |
| 1.14.13.7 | Mph | - |
Alcaligenes sp. IS-46 |
| 1.14.13.7 | multicomponent phenol hydroxylase | - |
Alcaligenes faecalis |
| 1.14.13.7 | multicomponent phenol hydroxylase | - |
Alcaligenes sp. IS-46 |
| 1.14.13.7 | phenol hydroxylase | - |
Alcaligenes faecalis |
| 1.14.13.7 | phenol hydroxylase | - |
Alcaligenes sp. IS-46 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.2 | 28 | - |
assay at | Alcaligenes faecalis |
| 1.14.13.7 | 28 | - |
- |
Alcaligenes faecalis |
| 1.14.13.7 | 28 | - |
assay at | Alcaligenes faecalis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.2 | 6.8 | 7 | - |
Alcaligenes faecalis |
| 1.14.13.7 | 6.8 | 7 | - |
Alcaligenes faecalis |
| 1.14.13.7 | 6.8 | 7 | assay at | Alcaligenes faecalis |
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