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Literature summary extracted from
- A hyaluronidase from Potamotrygon motoro (freshwater stingrays) venom: isolation and characterization (2008), Toxicon, 51, 1060-1067.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.35 | Cu2+ | - |
Potamotrygon motoro |  |
| 3.2.1.35 | Fe2+ | - |
Potamotrygon motoro | |
| 3.2.1.35 | heparin | - |
Potamotrygon motoro | |
| 3.2.1.35 | Mn2+ | - |
Potamotrygon motoro | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.35 | additional information | - |
additional information | Km (hyaluronan) (microgram/ml): 4.91, Vmax (hyaluronan): 2.02 U/min | Potamotrygon motoro |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 79000 | - |
gel filtration | Potamotrygon motoro |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | Potamotrygon motoro | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.35 | purified by gel filtration on Sephacryl S-100 and ion-exchange chromatography on SP-Sepharose. Procedure provides 376.4fold purification with a 2.94% yield | Potamotrygon motoro |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.35 | hyaluronan + H2O | - |
Potamotrygon motoro | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | monomer | 1 * 79000 Da, gel filtration and SDS-PAGE | Potamotrygon motoro |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | hyaluronidase | - |
Potamotrygon motoro |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 37 | - |
assay at | Potamotrygon motoro |
| 3.2.1.35 | 40 | - |
maximal activity | Potamotrygon motoro |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 30 | - |
enzyme is stable for at least 30 min when incubated at 20°C and 30°C | Potamotrygon motoro |
| 3.2.1.35 | 40 | - |
enzyme loses 70% of the activity at 40°C | Potamotrygon motoro |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 4.2 | - |
optimal pH | Potamotrygon motoro |
| 3.2.1.35 | 6 | - |
assay at | Potamotrygon motoro |
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