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Literature summary extracted from

  • Rustler, S.; Chmura, A.; Sheldon, R.A.; Stolz, A.
    Characterisation of the substrate specificity of the nitrile hydrolyzing system of the acidotolerant black yeast Exophiala oligosperma R1 (2008), Stud. Mycol., 61, 165-174.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.5 additional information expression is induced by growing the strain in the presence of different nitriles and /or complex or inorganic nitrogen sources. The highest nitrile hydrolysing activity is observed with cells grown with 2-cyanopyridine and NaNO3 Exophiala oligosperma

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.5 Exophiala oligosperma
-
-
-
3.5.5.5 Exophiala oligosperma R1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.5 2-hydroxy-3-butenenitrile + H2O
-
 Exophiala oligosperma 2-hydroxy-3-butenoic acid + NH3
-
 ?
3.5.5.5 3-cyanopyridine + 2 H2O 208% of the rate with benzonitrile, cell extract Exophiala oligosperma nicotinic acid + NH3
-
 ?
3.5.5.5 3-cyanopyridine + 2 H2O 208% of the rate with benzonitrile, cell extract Exophiala oligosperma R1 nicotinic acid + NH3
-
 ?
3.5.5.5 4-cyanopyridine + 2 H2O 512% of the rate with benzonitrile, cell extract Exophiala oligosperma 4-pyridinecarboxylic acid + NH3
-
 ?
3.5.5.5 acrylonitrile + H2O 121% of the rate with benzonitrile, cell extract Exophiala oligosperma acrylic acid + NH3
-
 ?
3.5.5.5 acrylonitrile + H2O 121% of the rate with benzonitrile, cell extract Exophiala oligosperma R1 acrylic acid + NH3
-
 ?
3.5.5.5 benzonitrile + 2 H2O
-
 Exophiala oligosperma benzoic acid + NH3
-
 ?
3.5.5.5 benzonitrile + 2 H2O
-
 Exophiala oligosperma R1 benzoic acid + NH3
-
 ?
3.5.5.5 additional information enzyme converts the isomers carrying a substituent in the meta-position with higher relative activities than the corresponding para- or ortho-substituted isomers. Aliphatic substrates such as acrylonitrile and 2-hydroxy-3-butenenitrile are also hydrolysed Exophiala oligosperma ?
-
 ?
3.5.5.5 additional information enzyme converts the isomers carrying a substituent in the meta-position with higher relative activities than the corresponding para- or ortho-substituted isomers. Aliphatic substrates such as acrylonitrile and 2-hydroxy-3-butenenitrile are also hydrolysed Exophiala oligosperma R1 ?
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O 22% of the rate with benzonitrile, cell extract Exophiala oligosperma phenylacetate + NH3
-
 ?
3.5.5.5 phenylacetonitrile + 2 H2O 22% of the rate with benzonitrile, cell extract Exophiala oligosperma R1 phenylacetate + NH3
-
 ?