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Literature summary extracted from

  • Giovannini, P.P.; Grandini, A.; Perrone, D.; Pedrini, P.; Fantin, G.; Fogagnolo, M.
    7alpha- and 12alpha-Hydroxysteroid dehydrogenases from Acinetobacter calcoaceticus lwoffii: a new integrated chemo-enzymatic route to ursodeoxycholic acid (2008), Steroids, 73, 1385-1390.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.159 NAD+
-
 Acinetobacter lwoffii

Application

EC Number Application Comment Organism
1.1.1.159 degradation new integrated chemo-enzymatic synthesis of ursodeoxycholic acid starting from sodium cholate by 7alpha- and 12alpha-hydroxysteroid dehydrogenases Acinetobacter lwoffii
1.1.1.176 degradation new integrated chemo-enzymatic synthesis of ursodeoxycholic acid starting from sodium cholate by 7alpha- and 12alpha-hydroxysteroid dehydrogenases Acinetobacter lwoffii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Acinetobacter lwoffii
-
-
-
1.1.1.176 Acinetobacter lwoffii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159 partially purified by ion-exchange chromatography Acinetobacter lwoffii
1.1.1.176 partially purified by ion-exchange chromatography Acinetobacter lwoffii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 0.035
-
 cell extract Acinetobacter lwoffii
1.1.1.159 0.11
-
 purified enzyme Acinetobacter lwoffii
1.1.1.176 0.027
-
 cell extract Acinetobacter lwoffii
1.1.1.176 0.3
-
 purified enzyme Acinetobacter lwoffii

Storage Stability

EC Number Storage Stability Organism
1.1.1.159 4°C, 7 days Acinetobacter lwoffii
1.1.1.176 4°C, 0.5 ml TEA-buffer, 3 days Acinetobacter lwoffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Acinetobacter lwoffii 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 ?
1.1.1.176 deoxycholic acid + NAD+
-
 Acinetobacter lwoffii 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7alpha-HSDH
-
 Acinetobacter lwoffii
1.1.1.176 12alpha-HSDH
-
 Acinetobacter lwoffii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NAD+
-
 Acinetobacter lwoffii