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Literature summary extracted from

  • Berg, I.A.; Kockelkorn, D.; Buckel, W.; Fuchs, G.
    A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea (2007), Science, 318, 1782-1786.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.120 4-hydroxybutanoyl-CoA Metallosphaera sedula
-
 but-3-enoyl-CoA + H2O
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A Metallosphaera sedula the enzyme is involved in the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle (autotrophic carbon dioxide assimilation pathway in archaea) 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.298 Metallosphaera sedula
-
-
-
4.2.1.120 Metallosphaera sedula
-
-
-
6.2.1.36 Metallosphaera sedula A4YGR1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.298 1.5
-
 65°C Metallosphaera sedula
4.2.1.120 0.04
-
 65°C Metallosphaera sedula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.298 malonate semialdehyde + NADPH
-
 Metallosphaera sedula 3-hydroxypropionate + NADP+
-
 ?
4.2.1.120 4-hydroxybutanoyl-CoA
-
 Metallosphaera sedula but-3-enoyl-CoA + H2O
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A
-
 Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?
6.2.1.36 3-hydroxypropanoate + ATP + coenzyme A the enzyme is involved in the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle (autotrophic carbon dioxide assimilation pathway in archaea) Metallosphaera sedula 3-hydroxypropanoyl-CoA + AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.298 malonate semialdehyde reductase (NADPH)
-
 Metallosphaera sedula
6.2.1.36 3-hydroxypropionyl-CoA synthetase (AMP-forming)
-
 Metallosphaera sedula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.36 55
-
 assay at Metallosphaera sedula

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.298 NADPH
-
 Metallosphaera sedula

General Information

EC Number General Information Comment Organism
1.1.1.298 physiological function enzyme is part of an autotrophic 3-hydroxypropionate/4-hydroxybutyrate carbon dioxide assimilation pathway in Metallospaera sedula. In the pathway, CO2 is fixed with acetyl-CoA/propionyl-CoA carboxylase as key carboxylating enzyme. One acetyl-CoA and two bicarbonate molecules are reductively converted via 3-hydroxypropionate to succinyl-CoA Metallosphaera sedula
4.2.1.120 physiological function enzyme is part of an autotrophic 3-hydroxypropionate/4-hydroxybutyrate carbon dioxide assimilation pathway in Metallospaera sedula. In the pathway, CO2 is fixed with acetyl-CoA/propionyl-CoA carboxylase as key carboxylating enzyme. One acetyl-CoA and two bicarbonate molecules are reductively converted via 3-hydroxypropionate to succinyl-CoA Metallosphaera sedula